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Biomedical Journal
Volume 29, Issue 3 (5-2025)
IBJ 2025, 29(3): 114-125 |
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Mousavi S, Rashidi M, Khedri A, Kouchak M, Salehi M, Zamani S et al . Atorvastatin-Infused Carboxymethyl Cellulose-Gelatin Hydrogel: A Synergistic Strategy for Enhanced Wound Healing and Skin Tissue Regeneration. IBJ 2025; 29 (3) :114-125
URL: http://ibj.pasteur.ac.ir/article-1-5043-en.html
URL: http://ibj.pasteur.ac.ir/article-1-5043-en.html
Seyed-Reza Mousavi 
, Mojtaba Rashidi , Azam Khedri , Maryam Kouchak , Majid Salehi , Sepehr Zamani , Ghorban Mohammadzadeh *
, Mojtaba Rashidi , Azam Khedri , Maryam Kouchak , Majid Salehi , Sepehr Zamani , Ghorban Mohammadzadeh *
Abstract:
Background: Skin tissue engineering is an innovative alternative to traditional methods for addressing skin injuries. This study aimed to synthesize a hydrogel consisting of carboxymethyl cellulose (CMC) and gelatin (Gel) containing atorvastatin (ATR), which has the potential to accelerate tissue regeneration and wound healing in an animal model.
Methods: Five unique formulations of hydrogel with different concentrations of ATR (0.1%, 0.5%, 1%, and 2% w/v) were synthesized using CMC-Gel. The structural characteristics of the hydrogels were assessed using SEM and FTIR spectroscopy. Additional evaluations included swelling behavior, degradability, ATR release, compatibility, hemolytic activity, and the toxic effects on the viability of NIH/3T3 fibroblast cells. Their therapeutic effectiveness of these hydrogels in enhancing wound healing was investigated in an animal model by making a full-thickness skin incision in Wistar rats.
Results: The synthesized CMC-Gel scaffolds had a porous structure with interconnected pores measuring 103 ± 8.74 μm, with the ability to enhance cell migration. The MTT analysis showed a dose-dependent relationship between ATR and cell proliferation, and its desirable concentration was 0.1% w/v. Furthermore, increased ATR concentrations were associated with decreased dressing capacity for hemostasis and coagulation. In vivo studies revealed that all the hydrogel-treated groups significantly outperformed the gas-treated control group in promoting wound closure rates. Remarkably, the CMC-Gel-ATR 0.1% group exhibited the highest rates of wound closure, re-epithelialization, and angiogenesis.
Conclusion: Our results demonstrated that CMC-Gel-ATR may be proposed as a desirable wound dressing for clinical application due to its unique physicochemical properties and comprehensive biocompatibility in in vitro and in vivo investigations.
Methods: Five unique formulations of hydrogel with different concentrations of ATR (0.1%, 0.5%, 1%, and 2% w/v) were synthesized using CMC-Gel. The structural characteristics of the hydrogels were assessed using SEM and FTIR spectroscopy. Additional evaluations included swelling behavior, degradability, ATR release, compatibility, hemolytic activity, and the toxic effects on the viability of NIH/3T3 fibroblast cells. Their therapeutic effectiveness of these hydrogels in enhancing wound healing was investigated in an animal model by making a full-thickness skin incision in Wistar rats.
Results: The synthesized CMC-Gel scaffolds had a porous structure with interconnected pores measuring 103 ± 8.74 μm, with the ability to enhance cell migration. The MTT analysis showed a dose-dependent relationship between ATR and cell proliferation, and its desirable concentration was 0.1% w/v. Furthermore, increased ATR concentrations were associated with decreased dressing capacity for hemostasis and coagulation. In vivo studies revealed that all the hydrogel-treated groups significantly outperformed the gas-treated control group in promoting wound closure rates. Remarkably, the CMC-Gel-ATR 0.1% group exhibited the highest rates of wound closure, re-epithelialization, and angiogenesis.
Conclusion: Our results demonstrated that CMC-Gel-ATR may be proposed as a desirable wound dressing for clinical application due to its unique physicochemical properties and comprehensive biocompatibility in in vitro and in vivo investigations.
Type of Study: Full Length/Original Article |
Subject:
Tissue Engineering and Cell Biology
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