Iranian
Biomedical Journal
Volume 29, Issue 3 (5-2025)
IBJ 2025, 29(3): 115-126 |
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Ethics code: IR.PII.REC.1400.079/IR.PII.REC.1399.082
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Mousavi S, Rashidi M, Khedri A, Kouchak M, Salehi M, Zamani S et al . Atorvastatin-Infused Carboxymethyl Cellulose-Gelatin Hydrogel: A Synergistic Strategy for Enhanced Wound Healing and Skin Tissue Regeneration. IBJ 2025; 29 (3) :115-126
URL: http://ibj.pasteur.ac.ir/article-1-5043-en.html
URL: http://ibj.pasteur.ac.ir/article-1-5043-en.html
Seyed-Reza Mousavi 
, Mojtaba Rashidi , Azam Khedri , Maryam Kouchak , Majid Salehi , Sepehr Zamani , Ghorban Mohammadzadeh *
, Mojtaba Rashidi , Azam Khedri , Maryam Kouchak , Majid Salehi , Sepehr Zamani , Ghorban Mohammadzadeh *
Abstract:
Background: Skin tissue engineering is an innovative alternative to traditional methods for addressing skin injuries. The aim of this study is to synthesize a hydrogel consisting of carboxymethyl cellulose (CMC) and gelatin (Gel) containing atorvastatin (ATR), which has the potential to accelerate tissue regeneration and wound healing in an animal model.
Methods: Five unique formulations of hydrogel with different concentrations of ATR (0.1%, 0.5%, 1%, and 2% w/v) were synthesized using CMC-Gel. The structural characteristics of hydrogels were assessed using Scanning Electron Microscope (SEM) and Fourier-Transform Infrared (FTIR) Spectroscopy. They were assessed for swelling behavior, degradability, ATR release, compatibility, hemolytic activity, and their toxic effects on the cell viability of NIH-3T3 fibroblasts. Their therapeutic effectiveness in enhancing wound healing was also investigated in an animal model by making a full-thickness skin incision in Wistar rats.
Results: The synthesized CMC-Gel scaffolds had a porous structure with interconnected pores measuring 103±8.74 μm with the ability to enhance cell migration. The MTT analysis showed that there was a dose-dependent relationship between atorvastatin and cell proliferation, and its desirable concentration was 0.1% w/v. Furthermore, increased ATR concentrations were associated with decreased dressing's capacity for hemostasis and coagulation. In vivo studies revealed that the all hydrogel-treated groups significantly outperformed the gas-treated control group in promoting wound closure rates. Remarkably, the CMC-Gel-ATR 0.1% group exhibited the highest rates of wound closure, re-epithelialization, and angiogenesis.
Conclusion: Our results demonstrated that CMC-Gel-ATR may be proposed as a desirable wound dressing for clinical application due to its unique physicochemical properties and comprehensive biocompatibility in an in-vitro and in-vivo investigations.
Methods: Five unique formulations of hydrogel with different concentrations of ATR (0.1%, 0.5%, 1%, and 2% w/v) were synthesized using CMC-Gel. The structural characteristics of hydrogels were assessed using Scanning Electron Microscope (SEM) and Fourier-Transform Infrared (FTIR) Spectroscopy. They were assessed for swelling behavior, degradability, ATR release, compatibility, hemolytic activity, and their toxic effects on the cell viability of NIH-3T3 fibroblasts. Their therapeutic effectiveness in enhancing wound healing was also investigated in an animal model by making a full-thickness skin incision in Wistar rats.
Results: The synthesized CMC-Gel scaffolds had a porous structure with interconnected pores measuring 103±8.74 μm with the ability to enhance cell migration. The MTT analysis showed that there was a dose-dependent relationship between atorvastatin and cell proliferation, and its desirable concentration was 0.1% w/v. Furthermore, increased ATR concentrations were associated with decreased dressing's capacity for hemostasis and coagulation. In vivo studies revealed that the all hydrogel-treated groups significantly outperformed the gas-treated control group in promoting wound closure rates. Remarkably, the CMC-Gel-ATR 0.1% group exhibited the highest rates of wound closure, re-epithelialization, and angiogenesis.
Conclusion: Our results demonstrated that CMC-Gel-ATR may be proposed as a desirable wound dressing for clinical application due to its unique physicochemical properties and comprehensive biocompatibility in an in-vitro and in-vivo investigations.
Type of Study: Full Length/Original Article |
Subject:
Tissue Engineering and Cell Biology
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