Iranian
Biomedical Journal
Volume 29, Issue 4 (4-2025)
IBJ 2025, 29(4): 228-235 |
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G. Gyurjyan Q, G. Poghosyan G, K. Gasparyan V. Apoferritin-Functionalized with Wheat Germ Agglutinin and Loaded with Antibiotic for Targeting Bacteria. IBJ 2025; 29 (4) :228-235
URL: http://ibj.pasteur.ac.ir/article-1-5039-en.html
URL: http://ibj.pasteur.ac.ir/article-1-5039-en.html
Abstract:
Background: The selective delivery of drugs to their targets prevents their possible side effects; hence, the development of selective transport systems is considered extremely promising. In the present study, we aimed to develop a drug delivery system for targeting bacteria.
Methods: Functionalization of apoferritin with wheat germ agglutinin (WGA), as a bacteria-recognizing lectin, was conducted. Afterwards, the complex was loaded with ampicillin. The affinity of the conjugate to Gram-positive bacteria, Bacillus subtilis, was evaluated by anisotropic silver nanoparticles conjugated with this complex, and its interaction with the bacteria was also assessed.
Results: The drug-delivery capabilities of the composite were evaluated. Results from the disk diffusion assay revealed that its bactericidal activity is 10-fold greater compared to free antibiotics. The effectiveness of the bactericidal effects of ampicillin-loaded ferritin was also confirmed in whole blood.
Conclusion. Lectin-conjugated and ampicillin-loaded apoferritin can be considered as effective drug delivery systems for targeting bacteria.
Methods: Functionalization of apoferritin with wheat germ agglutinin (WGA), as a bacteria-recognizing lectin, was conducted. Afterwards, the complex was loaded with ampicillin. The affinity of the conjugate to Gram-positive bacteria, Bacillus subtilis, was evaluated by anisotropic silver nanoparticles conjugated with this complex, and its interaction with the bacteria was also assessed.
Results: The drug-delivery capabilities of the composite were evaluated. Results from the disk diffusion assay revealed that its bactericidal activity is 10-fold greater compared to free antibiotics. The effectiveness of the bactericidal effects of ampicillin-loaded ferritin was also confirmed in whole blood.
Conclusion. Lectin-conjugated and ampicillin-loaded apoferritin can be considered as effective drug delivery systems for targeting bacteria.
Type of Study: Full Length/Original Article |
Subject:
Pharmaceutical Biotechnology
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