Iranian
Biomedical Journal
Volume 26, Issue 5 (9-2022)
IBJ 2022, 26(5): 366-373 |
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Salimi A, Amirimoghadam S, Bagheri F. Preparation, Optimization, and Investigation of Naringenin-Loaded Microemulsion for Topical Application. IBJ 2022; 26 (5) :366-373
URL: http://ibj.pasteur.ac.ir/article-1-3722-en.html
URL: http://ibj.pasteur.ac.ir/article-1-3722-en.html
Abstract:
Background: Flavonoids are a large group of phenolic compounds possessing anti-inflammatory and antioxidant effects. NAR is a flavonoid with various pharmacological properties. Using pharmaceutical compounds on skin is one of the routes of administration to achieve local and systemic effects. The aim of this study was to develop a topical formulation of NAR by the preparation of a NAR ME, which was further tested its skin permeability in rats.
Methods: Eight 0.5% NAR MEs were prepared by mixing appropriate amounts of surfactant (Tween 80 and Labrasol), cosurfactant (Capryol 90) and the oil phase (oleic acid-Transcutol P in a ratio of 1:10). The drug was dissolved in the oil phase. The physicochemical properties of MEs such as droplet size, viscosity, release, and skin permeability were assessed using Franz Cells diffusion.
Results: Based on the results, the droplet size of MEs ranged between 5.07 and 35.15 nm, and their viscosity was 164-291 cps. Independent factors exhibited a strong relationship with both permeability and drop size. The permeability findings revealed that the diffusion coefficient of NAR by the ME carrier increased compared to the drug saturation solution.
Conclusion: The most validated results were obtained for Jss and particle size. Optimal formulations containing MEs with Jss and particle sizes varying between minimum and maximum amounts are suitable for topical formulations of NAR.
Methods: Eight 0.5% NAR MEs were prepared by mixing appropriate amounts of surfactant (Tween 80 and Labrasol), cosurfactant (Capryol 90) and the oil phase (oleic acid-Transcutol P in a ratio of 1:10). The drug was dissolved in the oil phase. The physicochemical properties of MEs such as droplet size, viscosity, release, and skin permeability were assessed using Franz Cells diffusion.
Results: Based on the results, the droplet size of MEs ranged between 5.07 and 35.15 nm, and their viscosity was 164-291 cps. Independent factors exhibited a strong relationship with both permeability and drop size. The permeability findings revealed that the diffusion coefficient of NAR by the ME carrier increased compared to the drug saturation solution.
Conclusion: The most validated results were obtained for Jss and particle size. Optimal formulations containing MEs with Jss and particle sizes varying between minimum and maximum amounts are suitable for topical formulations of NAR.
Type of Study: Short Communication |
Subject:
Pharmaceutical Biotechnology
References
1. Harborne J, Williams CA. Advances in flavonoid research since 1992. Phytochemistry 2000; 55(6): 481-504. [DOI:10.1016/S0031-9422(00)00235-1]
2. Raj K, Shalini K. Flavonoids-a review of biological activities. Indian drug 1999; 36: 668-676.
3. Jayaraman J, Veerappan M, Namasivayam N. Potential beneficial effect of naringenin on lipid peroxidation and antioxidant status in rats with ethanol-induced hepatotoxicity. Journal of pharmacy and pharmacology 2009; 61(10): 1383-1390. [DOI:10.1211/jpp.61.10.0016]
4. Lisa J, Wilcox, Nica M. Borradaile, Murray W. Antiatherogenic properties of naringenin, a citrus flavonoid. nevapress. Cardiovascular drug reviews 1999; 17(2): 106-178. [DOI:10.1111/j.1527-3466.1999.tb00011.x]
5. Madsen HL, Andersen CM, Jorgensen LV, Skibsted LH. Radical scavenging by dietary flavonoids. A kinetic study of antioxidant efficiencies. European food research and technology 2000; 211(4): 240-246. [DOI:10.1007/s002170000189]
6. Yu J, Wang L, Walzem RL, Miller EG, Pike LM, Patil BS. Antioxidant activity of citrus limonoids, flavonoids, and coumarins. Journal of agricultural food chemistry 2005; 53(6): 2009-2014. [DOI:10.1021/jf0484632]
7. Khan AW, Kotta S, Ansari SH, Sharma RK, Ali J. Self-nanoemulsifying drug delivery system (SNEDDS) of the poorly water-soluble grapefruit flavonoid naringenin: design, characterization, in vitro and in vivo evaluation. Drug delivery 2015; 22(4): 552-561. [DOI:10.3109/10717544.2013.878003]
8. Touitou E. Drug delivery across the skin. Expert opinion on biologycal therapy 2002; 2(7): 723-733. [DOI:10.1517/14712598.2.7.723]
9. Thacharodi D, pharmaceutics KR-I journal of, 1994 undefined. Transdermal absorption of nifedipine from microemulsions of lipophilic skin penetration enhancers. International journal of pharmaceutics 1994; 111(3): 235-240. [DOI:10.1016/0378-5173(94)90346-8]
10. Martinez RM, Pinho-Ribeiro FA, Steffen VS, Silva TCC, Caviglione C V, Bottura C, Fonseca MJV, Vicentini FTMC, Vignoli JA, Baracat MM, Georgetti SR, Verri Jr WA, Casagrande R. Topical formulation containing naringenin: Efficacy against ultraviolet B irradiation-induced skin inflammation and oxidative stress in mice. PLoS One 2016; 11(1): 1-21. [DOI:10.1371/journal.pone.0146296]
11. Moghimipour E, Salimi A, Leis F. Preparation and evaluation of tretinoin microemulsion based on Pseudo-Ternary phase diagram. Advanced pharmacutical bulletin 2012; 2(2): 141-147.
12. Salimi A, Moghimipour E, Tavakolbekhoda N. Transdermal delivery of celecoxib through rat skin from various microemulsions. International research journal of pharmaceutical and applied sciences 2013; 3(4): 173-181.
13. Soliman SN, Abdel Malak NS, El-Gazayerly ON, Abdel Rahim AA. Formulation of microemulsion gel systems for transdermal delivery of celecoxib: In vitro permeation, anti-inflammatory activity and skin irritation tests. Drug Discoveries and therapeutics 2010; 4(6):459-71.
14. Salimi A, Zadeh BSM, Godazgari S, Rahdar A. Development and evaluation of azelaic acid-loaded microemulsion for transfollicular drug delivery through guinea pig skin: A mechanistic study. Advanced pharmacutical bulletin 2020; 10(2): 239-246. [DOI:10.34172/apb.2020.028]
15. Eastoe J, Paul A, Downer A, Steytler DC, Rumsey E. Effects of fluorocarbon surfactant chain structure on stability of water-in-carbon dioxide microemulsions. Links between aqueous surface tension and microemulsion stability. Langmuir 2002; 18(8): 3014-3017. [DOI:10.1021/la015576w]
16. Spicer PT, Small WB, Lynch ML, Burns JL. Dry powder precursors of cubic liquid crystalline nanoparticles (cubosomes). Journal of nanoparticle research 2002; 4: 297-311. [DOI:10.1023/A:1021184216308]
17. Krauel K, Girvan L, Hook S, Micron TR. Characterisation of colloidal drug delivery systems from the naked eye to Cryo-FESEM. Micron 2008; 38(8): 796-803. [DOI:10.1016/j.micron.2007.06.008]
18. Censi R, Martena V, Hoti E, Malaj L, Martino P Di. Permeation and skin retention of quercetin from microemulsions containing Transcutol P. Drug develupment and industrial Pharmacy 2012; 38(9): 1128-1133. [DOI:10.3109/03639045.2011.641564]
19. KamranfarM P, Jamialahmadi M. Effect of surfactant micelle shape transition on the microemulsion viscosity and its application in enhanced oil recovery processes. Journal of molecular liquids 2014; 198: 286-291. [DOI:10.1016/j.molliq.2014.07.009]
20. Gupta S, Chavhan S, Krutika KS. Self-nanoemulsifying drug delivery system for adefovir dipivoxil: design, characterization, in vitro and ex vivo evaluation. Colloids and surfaces A: physicochemical and engineering aspects 2011; 392(1): 145-155. [DOI:10.1016/j.colsurfa.2011.09.048]
21. Tsai MJ, Huang Y B, Fang JW, Fu YS, Wu PC. Preparation and evaluation of submicron-carriers for naringenin topical application. International journal of pharmaceutics 2015; 481(1-2): 84-90. [DOI:10.1016/j.ijpharm.2015.01.034]
22. Naik A, Pechtold LA.R.M, Potts RO, Guy RH. Mechanism of oleic acid-induced skin penetration enhancement in vivo in humans. Journal of controlled release 1995; 37(3): 299-306. [DOI:10.1016/0168-3659(95)00088-7]
23. Williams AC, Barry BW. Penetration enhancers. Advanced drug delivery reviews 2012; 64: 128-137. [DOI:10.1016/j.addr.2012.09.032]
24. Abbasi N, Khalighi Z, Eftekhari Z, Bahmani M. Extraction and phytoanalysis of chemical compounds of Eucalyptus globulus leaf native to Dehloran, Ilam province, Iran by HS-SPME and GC-MS. Advances in animal and veterinary sciences 2020; 8(6): 647-652. [DOI:10.17582/journal.aavs/2020/8.6.647.652]
25. Bahmani M, Jalilian A, Salimikia I, Shahsavari S, Abbasi N. Phytochemical screening of two Ilam native plants Ziziphus nummularia (Burm. f.) Wight & Arn. and Ziziphus spina-christi (Mill.) Georgi using HS-SPME and GC-MS spectroscopy. Plant science today 2020; 6; 7(2): 275-80. [DOI:10.14719/pst.2020.7.2.714]
26. Eftakhari Z, Patra I, Hamza TA, Adhab AH, Hachim Sk. Evaluation of the Total Antioxidant Capacity of Bitter and Sweet Varieties of Ferula assa-foetida andBunium persicum. Advancements in life sciences 2022; 9(3): 363-367.
27. AL-Ethawi MB, AL-Taae HH. First record at molecular level for Rhizoctonia solani causing rot root on aleo vera plants in Iraq. Caspian journal of environmental sciences, 2022; 1-11.
28. Mashabela N M, Otang-Mbeng W. Evaluating the effect of fertilizers on physiological growth, chemical, bioactive components and secondary metabolites in vigna unguiculata. Plant biotechnology persa 2022; 4(1): 37-49. [DOI:10.52547/pbp.4.1.6]
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