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Abstract:  
Background: We investigated the anti-nociceptive effect of Cuminum cyminum L. (cumin), which is an herbaceous plant with analgesic properties, and its biosynthesized gold nanoparticles (cumin-AuNPs). Methods: Cumin extract (E) and cumin-AuNPs (GN+E) were prepared and administered intraperitoneally at concentrations of 200 (E200 and GN+E200, respectively), 500 (E500 and GN+E500, respectively), and 1000 (E1000 and GN+E1000, respectively) mg/ml to twenty-seven male rats. Ultraviolet-visible (UV–Vis) spectroscopy and Atomic Force Microscopy (AFM) were applied for cumin-AuNPs synthesis confirmation. The nociceptive behavior was assessed using the Tjolsen method. IL-6 serum levels were measured using ELISA. Results: UV-Vis spectroscopy showed a peak absorption of cumin-AuNPs at 515 nm, and AFM showed a size of about 40nm. Three different concentrations of cumin extract had no significant effect on acute and chronic formalin-induced nociceptive behavior. GN+E200 (46.00±10.59) showed a significant acute anti-nociceptive effect compared to the control (98.66±4.91, P=0.029) and Sodium Salicylate (SS300) (98.33±20.30, P=0.029) groups. Also, GN+E500 (42.00±11.84) significantly reduced acute nociceptive behavior compared to control (98.66±4.91, P=0.019), SS300 (98.33±20.30, P=0.020) and GN+E1000 (91.00±26.00, P=0.040) groups. IL-6 serum levels reduced significantly in GN+E500 (24.65±10.38, P=0.002) and SS300 (33.08±1.68, P=0.039) compared to the control (46.24±3.02) groups. There was no significant difference in serum levels of interleukin 6 between any of the experimental and the control groups. Chronic nociceptive behavior was significantly lower in the SS300 (255.33±26.30) compare to E200 (477.00 ±47.29, P=0.021), E500 (496.25 ±46.29, P=0.013) and GN+E500 (437.00 ±118.03, P=0.032) groups. Conclusion: These results suggest the potential effects of cumin-AuNPs on formalin-induced nociceptive behavior that is independent of serum levels of IL-6.
     
Type of Study: Full Length | Subject: Pharmaceutical Biotechnology

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