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The yeast cell wall consists of polysaccharides and proteins, which are linked to mannan oligosaccharides and known as the mannoprotein complex. In an attempt to obtain highly purified mannan oligosaccharides from Kluyveromyces marxianus, the present study investigated the extraction and deproteinization of mannoprotein. The mannan oligosaccharide loss, deproteinization percentage, and chemical composition were compared by applying the Sevage, TCA, and HCl methods. The results demonstrated that the HCl method had the highest deproteinization percentage and a slightly greater percentage of mannan oligosaccharide loss than the other two methods. The yield and purity of the crude mannoprotein were 8.42 ± 0.06% and 67.4 ± 1.3%, respectively, which, in turn, reached to the values of 6.84 ± 0.3% and 75.01 ± 0.48% after the removal of the free protein by the HCl method. The Sevage method exceeded the other two methods in the yield and purity of mannan oligosaccharides.

Type of Study: Full Length | Subject: Related Fields

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