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Analysis of NSP4 Gene and Its Association with Genotyping of Rotavirus Group A in Stool Samples
Ali Teimoori , Mehrab Nejati, Saeedeh Ebrahimi, Manoochehr Makvandi, Milad Zandi, Azarakhsh Azaran
Abstract:  

Background: Non-structural protein 4 (NSP4) is a critical protein for rotavirus (RV) replication and assembly. This protein has multiple domains and motifs that predispose its function and activity. NSP4 has a sequence divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of NSP4 have been identified, and E1 and E2 have been shown to be the most common genotypes in human. Methods: The gene and protein sequence of NSP4 in RV-positive samples were inspected with the aim of NSP4 genotyping and variation analysis in viroporin and other domains. P and G typing of RV samples have been carried out by WHO primers using a semi-multiplex PCR method. Non-typeable RV samples were amplified by conserved primers and sequenced. Results: In viroporin and enterotoxin, conserved sequence was detected, and amino acids substitution with the same biochemical properties was found. Conclusion: Moreover, association of NSP4 genotype with P or G genotyping G1/G9 correlates with E1 genogroups. In electrophoretyping of RV, E2 genotype had short pattern when compared to E1.

Keywords: NSP4, Rotavirus, Genotyping
Full-Text [PDF 671 kb]      
Type of Study: Full Length | Subject: Molecular Microbiology
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PMID: 28693095


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Teimoori A, Nejati M, Ebrahimi S, Makvandi M, Zandi M, Azaran A. Analysis of NSP4 Gene and Its Association with Genotyping of Rotavirus Group A in Stool Samples . IBJ. 2017;
URL: http://ibj.pasteur.ac.ir/article-1-2115-en.html
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