Facile Green Synthesis of Iron Oxide Nanoparticles and Their Impact on Cytotoxicity, Antioxidative Properties and Bactericidal Activity

Shah, Sejal S.; Turakhia, Bhavika P.; Purohit, Nihar; Kapadiya, Khushal M.; Sahoo, Chita R.; Prajapati, Akhilesh

doi:10.61186/ibj.4061

Facile Green Synthesis of Iron Oxide Nanoparticles and Their Impact on Cytotoxicity, Antioxidative Properties and Bactericidal Activity

Authors

Sejal S. Shah ¹

Bhavika P. Turakhia ¹

Nihar Purohit ²

Khushal M. Kapadiya ³

Chita R. Sahoo ⁴

Akhilesh Prajapati ²

¹ Department of Bioinformatics, Faculty of Technology, Marwadi University, Rajkot, Gujarat, India

² Department of Life Sciences, Biotechnology Division, School of Science, GSFC University, Vadodara-391750, Gujarat, India

³ School of Science, Department of Chemistry, RK University, Rajkot, Gujarat, India

⁴ Central Research Laboratory, Institute of Medical Sciences & SUM Hospital, Siksha O Anusandhan Deemed to be University, Bhubaneswar 751003, Odisha, India

10.61186/ibj.4061

Abstract

Background: Bioreductive processes are quite potent, effective and affordable for the synthesis of green nanoparticles (NPs), as compared to the physical and chemical methods. The present study aimed to evaluate the bactericidal, antioxidative and anticancer activity of turmeric rhizome-iron oxide nanoparticles (FeONPs) derived from the turmeric rhizome (Curcuma amada) using ferric chloride as a precursor.

Methods: With focusing on the manufacture of FeONPs via green approach, we characterized the NPs using FTIR, FT-Vis, DLS, and UV-Vis spectroscopy. The produced particles were tested for antibacterial, antioxidant, and anticancer properties. The synthesized NPs were also examined using the MDA-MB-231 human epithelial breast cancer cell line and NCI-60 cancer cell lines.

Results: The antioxidant activity of TR-FeONPs was concentration-dependent. The scavenging activity of TR-FeONPs was 76.09% at a concentration of 140 µg/ml. Using different concentrations of TR-FeONPs in the MTT assay against the MDA-MB-231 cell line indicated a reduction of less than 50% in cell viability at 125 µg/ml. Moreover, TR-FeONPs exhibited an effective bactericidal property. The gTR-FeONPs synthesized bioreductively were found to be effective in renal cancer, UO-31 cell line, with GI₅₀ value of 66.64%.

Conclusion: Our study showcases a sustainable method based on green chemistry principles to produce FeONPs utilizing turmeric rhizome. We anticipate that the FeONPs produced through this biosynthesis process could serve as a promising drug delivery system in cancer treatment

and as an effective antimicrobial agent against various diseases.

Keywords

antioxidants

Bactericidale activity

Green synthesis

MDA-MB-231cells

Molecular Docking