Optimal Extraction and Deproteinization Method for Mannoprotein Purification from Kluyveromyces marxianus

Authors
Ashraf Hajhosseini 1
Anoosheh Sharifan 1
Zohreh Eftekhari 2
Ariana Alavi 3
Delaram Doroud 3
1 Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
3 Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran
10.61186/ibj.27.5.320
Abstract
Background: Mannoproteins, mannose-glycosylated proteins, play an important role in biological processes and have various applications in industries. Several methods have been already used for the extraction of mannoproteins from yeast cell-wall. The aim of this study was to evaluate the extraction and deproteinization of mannan oligosaccharide from the Kluyveromyces (K.) marxianus mannoprotein.

Methods: To acquire crude mannan oligosaccharides, K. marxianus mannoproteins were deproteinized by the Sevage, trichloroacetic acid, and hydrochloric acid (HCL) methods. Total nitrogen, crude protein content, fat, carbohydrate and ash content were measured according to the monograph prepared by the meeting of the Joint FAO/WHO Expert Committee and standard. Mannan oligosaccharide loss, percentage of deproteinization, and chemical composition of the product were assessed to check the proficiency of different methods.

Results: Highly purified (95.4%) mannan oligosaccharide with the highest deproteinization (97.33 ± 0.4%) and mannan oligosaccharide loss (25.1 ± 0.6%) were obtained following HCl method.

Conclusion: HCl, was the most appropriate deproteinization method for the removal of impurities. This preliminary data will support future studies to design scale-up procedures.
Keywords
Kluyveromyces marxianus
Mannan oligosaccharides
Downstream extraction process
Iranian Biomedical Journal
Volume 27, Issue 5 - Serial Number 110
September and October 2023
Pages 320-325