Woodchuck Hepatitis Virus Post-Transcriptional Regulation Element (WPRE) Promotes Anti-CD19 BiTE Expression in Expi293 Cells

Authors
Reza Moazzami 1
Hasan Mirzahoseini 1
Leila Nematollahi 1
Farzaneh Barkhordari 1
Mozhgan Raigani 1
Fatemeh Hajari Taheri 2
Fereidoun Mahbudi 2
Fatemeh Davami 1
1 Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
2 Department of Immunology, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran
10.52547/ibj.25.4.275
Abstract
Background: Bispecific antibodies represent an important class of monoclonal antibodies (mAbs), with great therapeutic potentials due to their ability to target simultaneously two distinct epitopes. The generation of functional bispecific antibodies with the highest possible yields is particularly critical for the production of these compounds on industrial scales. Anti-CD3 × CD19 bispecific antibody (bsAb) is a bispecific T-cell engager currently used for treating ALL. Herein, we have tried to optimize the expression level of this antibody in mammalian hosts. Methods: Woodchuck hepatitis virus post-transcriptional regulation (WPRE) sequence was incorporated at the 3’ end of the expression cassette. This modification resulted in a notable about two-fold increase in the expression of the bsAb in the Expi293 cell line. Results & Conclusion: Follow-up flow cytometry analysis demonstrated the binding properties of the produced antibody at acceptable levels, and in vitro bioactivity assays showed that this product is potent enough for targeting and destroying CD19-positive cells. Our findings show that WPRE enhances the expression of this type of bispecific mAbs in human embryonic kidney-293 family cell lines. This approach can be used in biopharma industry for the mass production of anti-CD3 × CD19 bispecific antibody.
Keywords
Acute lymphoblastic leukemia
Bispecific antibodies
monoclonal antibody
Iranian Biomedical Journal
Volume 25, Issue 4 - Serial Number 98
July and August 2021
Pages 275-283