Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber

Authors
Atena Shapourzadeh 1
Seyed Mohammad Atyabi 2
Shiva Irani 3
Hadi Bakhshi 4
1 Department of Biochemistry, Faculty of Basic Science, Islamic Azad University Damghan Branch, Damghan, Iran
2 Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran 13164, Iran
3 Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Universitatsstraße Bayreuth, Germany-Universitatsstraße Bayreuth, Germany
10.29252/ibj.24.4.236
Abstract
Background: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. Methods: In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. Results: The results of Fourier Transform Infrared Spectroscopy confirmed CMC (20%) graft on PCL scaffold. The Thiazolyl blue tetrazolium bromide assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of adipose-derived stem cells (ADSCs) to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. Conclusion: Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.
Keywords
carboxymethyl chitosan
Mesenchymal stem cells
Tissue engineering
Iranian Biomedical Journal
Volume 24, Issue 4 - Serial Number 92
July and August 2020
Pages 236-242