Alu DNA Polymorphism of Human Tissue Plasminogen Activator (tPA) Gene in Diabetic Jordanian Patients Patients

Authors
Salem R. Yasin 1
Hussam H. AlHawari 2
Abeer A. Alassaf 3
Maysa M. Khadra 4
Zainab A. Al-Mazaydeh 5
Ala'a F. Al-Emerieen 6
Lubna H. Tahtamouni 5
1 1Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan
2 Department of Internal Medicine, Faculty of Medicine, University of Jordan, Amman, Jordan
3 Department of Pediatric, Faculty of Medicine, University of Jordan, Amman, Jordan
4 Department of Obstetrics and Gynecology, Faculty of Medicine, University of Jordan, Amman, Jordan
5 Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan
6 Department of Service Courses, Faculty of Science, Zarqa Private University, Zarqa, Jordan
10.29252/ibj.23.6.423
Abstract
Background: Hypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimed to address the polymorphic nature of Alu DNA fragment in the human tissue plasminogen activator gene within diabetes mellitus (DM) Jordanian patients. Methods: Genomic DNA was isolated from 76 DM patients and 60 non-diabetic Jordanian individuals, and the Alu fragment was amplified using PCR. Results: The results showed that 80% of the non-diabetic Jordanian subjects were homozygotes for the deletion of the Alu fragment (Alu-/-), 16.7% were homozygotes for its insertion (Alu+/+), and 3.3% were heterozygotes (Alu+/-). Besides, 36.8% of the diabetic patients exhibited the Alu-/- or Alu+/- genotype, and 26.3% were Alu+/+. The Alu-/- genotype occurred less frequently in the diabetic individuals. Conclusion: The high frequency of the Alu-/- genotype constitutes a protective deletion with respect to DM within the normal subjects.
Keywords
Alu
Diabetes Mellitus
Polymorphism
Iranian Biomedical Journal
Volume 23, Issue 6 - Serial Number 88
November and December 2019
Pages 423-428