Clinical and in vitro Study of Novel Long Non-Coding RNA lncUSMycN in Breast Cancer Cancer

Ravanbakhsh Gavgani, Reyhaneh; Babaei, Esmaeil; Hosseinpourfeizi, Mohammad Ali; Fakhrjou, Ashraf; Montazeri, Vahid

doi:10.29252/ibj.23.5.1

Clinical and in vitro Study of Novel Long Non-Coding RNA lncUSMycN in Breast Cancer Cancer

Authors

Reyhaneh Ravanbakhsh Gavgani ¹

Esmaeil Babaei ¹

Mohammad Ali Hosseinpourfeizi ¹

Ashraf Fakhrjou ²

Vahid Montazeri ³

¹ Department of Biological Sciences, School of Natural Sciences, University of Tabriz, Tabriz, Iran

² Department of Pathology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

³ Department of Thoracic Surgery, Noor-Nejat Hospital, Tabriz, Iran

10.29252/ibj.23.5.1

Abstract

Background: Despite recent advances in diagnosis and treatment, breast cancer remains a leading cause of death in women worldwide. Long non-coding RNAs are a new class of RNA molecules that have been shown to participate in tumorigenesis. The aim of this study was to investigate the expression of lncUSMycN in tumor samples and to evaluate its potential role in the breast cancer cell line. Methods: Real-time polymerase chain reaction was employed to assess lncUSMycN expression in breast tumor tissues and cancer cell lines. Furthermore, small interfering RNA was used to knockdown lncUSMycN. Results: The data showed the significant up-regulation of lncUSMycN in tumor tissues compared to non-tumor specimens (95% CI, p = 0.002). Receiver operating characteristic (ROC) curve analysis demonstrated the biomarker potential of lncUSMycN (ROCAUC = 0.70, p < 0.001) for invasive breast ductal carcinoma. Furthermore, lncUSMycN knockdown induced apoptosis and suppressed cellular migration in breast cancer cells (p < 0.01). Conclusion: The findings highlight the pivotal role of lncUSMycN in tumorigenesis, providing a new potential target for breast cancer therapy.

Keywords

Breast cancer

Real-Time Polymerase Chain Reaction

siRNA