Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model

Abbaszadeh, Hojjat Allah; Tiraihi, Taki; Sadeghi, Yousef; Delshad, Ali Reza; Sadeghizadeh, Majid; Taheri, Taher; Noori-Zadeh, Ali

doi:10.29252/ibj.22.4.246

Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model

Authors

Hojjat Allah Abbaszadeh ¹

Taki Tiraihi ²

Yousef Sadeghi ¹

Ali Reza Delshad ³

Majid Sadeghizadeh ⁴

Taher Taheri ⁵

Ali Noori-Zadeh ⁶

¹ Hearing Disorders Research Center and Department of Biology and Anatomical Sciences, School of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

² Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

³ Department of Anatomy, Shahed University, Tehran, Iran

⁴ Department of Genetics, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran

⁵ Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran

⁶ Department of Clinical Biochemistry, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran

10.29252/ibj.22.4.246

Abstract

Background: Oligodendrocyte cell death is among the important features of spinal cord injury, which appears within 15 min and occurs intensely for 4 h after injury, in the rat spinal contusion model. Accordingly, the number of oligodendrocytes progressively reduced within 24 h after injury. Administration of oligodendrocyte-like cells (OLCs) into the lesion area is one of the approaches to counterbalance this condition. Methods: Bone marrow stromal cells were transdifferentiated into neurospheres and then into neural stem cells and later were differentiated into OLCs using triiodothyronine and transplanted into the spinal cord contusion rats. The post-injury functional recovery was explored and compared with the control group using Basso-Beattie-Bresnahan and narrow beam behavioral tests. At the end of 12^th week, spinal cord segments T12-L1 were histomorphologically studied by immunohistochemistry. Results: Motor improvement was more obvious during 2^nd to 4^th weeks and got less prominent during 4^th to 12^th weeks. Histomorphometric findings indicated that cavity formation decreased in epicenter of transplantation area in experimental groups in comparison with the control groups. Conclusion: The findings obtained in the present study showed that OLC therapy is a potential approach in the treatment of spinal cord traumatic injuries.

Keywords

Spinal Cord Injury

Myelin basic protein

Oligodendrocyte