In vitro Study of SPIONs-C595 as Molecular Imaging Probe for Specific Breast Cancer (MCF-7) Cells Detection

Moradi Khaniabadi, Pegah; Shahbazi-Gahrouei, Daryoush; Malik  Shah Abdul Majid, Amin; Suhaimi Jaafar, Mohammad; Moradi  Khaniabadi, Bita; Shahbazi-Gahrouei, Saghar

doi:10.18869/acadpub.ibj.21.6.360

In vitro Study of SPIONs-C595 as Molecular Imaging Probe for Specific Breast Cancer (MCF-7) Cells Detection

Authors

Pegah Moradi Khaniabadi ¹

Daryoush Shahbazi-Gahrouei ²

Amin Malik Shah Abdul Majid ³

Mohammad Suhaimi Jaafar ¹

Bita Moradi Khaniabadi ⁴

Saghar Shahbazi-Gahrouei ⁵

¹ School of Physics, Universiti Sains Malaysia11800, Pulau Penang, Malaysia

² Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

³ School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia;

⁴ Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran

⁵ School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

10.18869/acadpub.ibj.21.6.360

Abstract

Background: Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7). Methods: The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy. The selectivity of the nanoprobe for breast cancer cells (MCF-7) was obtained by Prussian blue, atomic emission spectroscopy, and MRI relaxometry. Results: The in vitro MRI showed that T₂ relaxation time will be reduced 76% when using T₂-weighed magnetic resonance images compared to the control group (untreated cells) at the dose of 200 μg Fe/ml, as the optimum dose. In addition, the results showed the high uptake of nanoprobe into MCF-7 cancer cells. Conclusion: The SPIONs-C595 nanoprobe has potential for the detection of specific breast cancer.

Keywords

nanoparticles

Molecular imaging

MCF-7 cells

monoclonal antibody