A Novel Defensin-Like Peptide Associated with Two Other New Cationic Antimicrobial Peptides in Transcriptome of the Iranian Scorpion Venom

Authors
Masoumeh Baradaran 1
Amir Jalali 2
Maryam Naderi soorki 3
Hamid Galehdari 1
1 Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Pharmacology and Toxicology, Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Department of Genetics, Sciences Faculty, Shahid Chamran University, Ahvaz, Iran
10.18869/acadpub.ibj.21.3.190
Abstract
Introduction: Scorpion venom is a source of bioactive peptides, and some antimicrobial peptides (AMPs) have been found in the venom gland of scorpions. Therefore, the discovery of new anti-infective agents is an essential need to overcome the problem of antibiotic resistance of clinical isolates. Here, we describe three new cationic AMPs, including meuVAP-6, meuAP-18-1, and meuPep34 from the venom gland of the Iranian scorpion, Mesobuthus eupeus. Methods: The cDNA sequences encoding all the three peptides were obtained from the cDNA library of scorpion venom gland and were deposited in the GenBank database. Results: MeuVAP-6 and meuAP-18-1 are non-disulphide-bridged antimicrobial peptides, while meuPep34 is a cysteine-rich defensin-like peptide. Discussion: All three identified AMPs are rich in arginine and tryptophan. The overall results from the length, net charge, and hydrophobicity index suggested that meuPep34 could be the most active AMPs with the potential ability of biofilm inhibition. The data from molecular characterization of identified AMPs can provide a platform for further investigations in the drug design.
Keywords
Antimicrobial cationic peptides
Defensin-like peptide
Mesobuthus eupeus
Iranian Biomedical Journal
Volume 21, Issue 3 - Serial Number 73
May and June 2017
Pages 190-196