A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System

Authors
Narjes Shokrollahi
Delavar Shahbazzadeh
Kamran Pooshang-Bagheri
Mahdi Habibi-Anbouhi
Ali Jahanian-Najafabadi
Mahdi Behdani
10.7508/ibj.2016.03.008
Abstract
Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. Results: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. Conclusion: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
Keywords
Baculovirus
Sf9
Transfection
Iranian Biomedical Journal
Volume 20, Issue 3 - Serial Number 68
July and August 2016
Pages 182-186