The Effect of Human Platelet-Rich Plasma on Adipose-Derived Stem Cell Proliferation and Osteogenic Differentiation

Authors
Sima Tavakolinejad
Mohsen Khosravi
Baratali Mashkani
Alireza Ebrahimzadeh Bideskan
Nasser Sanjar Mossavi
Seyyed Mohammad Reza Parizadeh
Daryoush Hamidi Alamdari
Mashhad University of Medical Sciences
10.6091/ibj.13012.2014
Abstract
Background: The cultured mesenchymal stem cells (MSC) have been used in many clinical trials however, there are still some concerns about the cultural conditions. One concern is related to the use of FBS as a widely used xenogeneic supplement in the culture system. Human platelet-rich plasma (hPRP) is a candidate replacement for FBS. In this study, the effect of hPRP on MSC proliferation and osteogenic differentiation has been evaluated. Methods: Human adipose-derived stem cells (hADSC) were expanded. Cells from the third passage were characterized by flow cytometric analysis and used for in vitro experiments. Resazurin and alizarin red stains were used for cell proliferation and osteogenic differentiation assays, respectively. Results: Treatment with hPRP resulted in a statistically significant increase in cell proliferation compare to the negative control group (P<0.001). Cell proliferation in the 15% hPRP group was also significantly higher than that in the 10% hPRP group (P<0.05). Additionally, it caused less osteogenic differentiation of the hADSC compared to the FBS (P<0.001), but in comparison to negative control, it caused acceptable mineralization (P<0.001). Conclusion: These findings indicate that hPRP not only improves the proliferation but also it can be a suitable substitution in osteogenic differentiation for clinical purposes. However, the clinical application value of hPRP still needs more investigation.
Keywords
Platelet-rich plasma
Adipose tissue
Stem cells
Cell Differentiation
Cell proliferation
Iranian Biomedical Journal
Volume 18, Issue 3 - Serial Number 60
July and August 2014
Pages 151-157