DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active.
Bouzari, S. & Rechinsky, V. O. (1998). Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector. Iranian Biomedical Journal, 2(2), 79-82.
MLA
Bouzari, S., & Rechinsky, V. O. "Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector", Iranian Biomedical Journal, 2, 2, 1998, 79-82.
HARVARD
Bouzari S., Rechinsky V. O. (1998). 'Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector', Iranian Biomedical Journal, 2(2), pp. 79-82.
CHICAGO
S. Bouzari & V. O. Rechinsky, "Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector," Iranian Biomedical Journal, 2 2 (1998): 79-82,
VANCOUVER
Bouzari S., Rechinsky V. O. Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector. Iranian Biomedical Journal. 1998;2(2):79-82.
Iranian Biomedical Journal