A sensitive high performance liquid chromathography (HPLC) analytical procedure was developed for the quantitive determination of trimethoprim (TM* and sulphamethoxazole (SM) in commercial dosage forms. C18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate as aqueous phase and acetonitrile with 0.4% triethylamine as organic phase. The drugs were quantified at flow-rate of 1.2 ml/min, with ultraviolet detection at 260 nm. The minimum detectable quantities in assays were 100 ng/ml for SM and 75 ng/ml for TM. The method is well suited to routine application and adequate sensitivity with precision.
Rezaee, A., Behzadian Nejad, Q. & Kebriaeezadeh, A. (2000). Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography. Iranian Biomedical Journal, 4(Issue 2 & 3), 75–78.
MLA
Rezaee, A., Behzadian Nejad, Q., & Kebriaeezadeh, A. "Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography", Iranian Biomedical Journal, 4, Issue 2 & 3, 2000, 75–78.
HARVARD
Rezaee, A., Behzadian Nejad, Q., & Kebriaeezadeh, A. (2000). 'Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography', Iranian Biomedical Journal, 4(Issue 2 & 3), pp. 75–78.
CHICAGO
Rezaee, A., Behzadian Nejad, Q. & Kebriaeezadeh, A., "Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography." Iranian Biomedical Journal, 4 Issue 2 & 3 (2000): 75–78,
VANCOUVER
Rezaee A., Behzadian Nejad Q., Kebriaeezadeh A. Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography. Iranian Biomedical Journal. 2000;4(Issue 2 & 3):75–78.
