Volume 27, Issue 1 (1-2023)                   IBJ 2023, 27(1): 66-71 | Back to browse issues page

PMID: 36624700
PMCID: PMC9971713

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Background: The role of inflammatory cytokines, such as tumor necrosis- alpha (TNF-α) and IL-8, in gastric carcinogenesis has been investigated, but their impact remains to be further elucidated.
Methods: In this study, we measured the serum concentrations of these cytokines and H. pylori serostatus in dyspeptic patients, presenting with normal mucosa (NM = 53), chronic gastritis (CG = 94), and gastric cancer (GC = 82), by ELISA.
Results: Moderate levels of TNF-α were detected in the NM group (19.9 ± 19.5 pg/ml), which were nearly doubled in patients with CG (35.7 ± 28.0 pg/ml) and drastically declined in GC patients (1.8 ± 5.9 pg/ml). The serum levels of IL-8, however, were not statistically different amongst these three groups.
Conclusion: TNF-α serum concentration seemed to undergo up- and downregulation, when moving from NM to CG and from CG to GC, respectively. If confirmed in a prospective study, this cytokine can behave as a serum indicator of gastric inflammation and malignant transformation.
Type of Study: Short Communication | Subject: Cancer Biology

1. Rawla P, Barsouk A. Epidemiology of gastric cancer: global trends, risk factors and prevention. Przegla̜d gastroenterologiczny 2019; 14(1): 26. [DOI:10.5114/pg.2018.80001]
2. Correa P, Piazuelo MB. The gastric precancerous cascade. Journal of digestive diseases 2012; 13(1): 2-9. [DOI:10.1111/j.1751-2980.2011.00550.x]
3. van Loo G, Bertrand MJM. Death by TNF: a road to inflammation. Nature reviews. immunology 2022; 15: 1-15. [DOI:10.1038/s41577-022-00792-3]
4. Najdaghi S, Razi S, Rezaei N. An overview of the role of interleukin-8 in colorectal cancer. Cytokine 2020; 135: 155205. [DOI:10.1016/j.cyto.2020.155205]
5. Bounder G, Jouimyi MR, Boura H, Touati E, Michel V, Badre W, Jouhadi H, Kadi M , Eljihad M, Benomar H, Kettani A, Lebrazi H, Maachi F. Associations of the -238(G/A) and -308(G/A) TNF-α Promoter Polymorphisms and TNF-α Serum Levels with the Susceptibility to Gastric Precancerous Lesions and Gastric Cancer Related to Helicobacter pylori Infection in a Moroccan Population. APJCP 2020; 21(6): 1623-1629. [DOI:10.31557/APJCP.2020.21.6.1623]
6. Sun X, Xiang C, Wu J, Dong W, Zhan Z, Wang R, Zhang JF. Relationship between serum inflammatory cytokines and lifestyle factors in gastric cancer. Molecular and clinical oncology 2019; 10(3): 401-414. [DOI:10.3892/mco.2019.1804]
7. Aksoy EK, Akpınar MY, Doğan Ö, Göktaş Z, Sapmaz FP, Şimşek GG, Uzman M, Nazlıgül Y. Clinical Significance of Serum Vascular Endothelial Growth Factor, Pigment Epithelium-Derived Factor, Tumor Necrosis Factor Alpha, and Progranulin Levels in Patients with Gastric Cancer and Gastric Precancerous Lesions. Journal of gastrointestinal cancer 2019; 50(3): 537-542. [DOI:10.1007/s12029-019-00251-8]
8. Kabir S, Grant C, Daar AS. Serum levels of interleukin-1, interleukin-6 and tumour necrosis factor-alpha in patients with gastric carcinoma. Cancer letters 1995; 95(1-2): 207-12. [DOI:10.1016/0304-3835(95)03895-4]
9. Shimamura H, Iwagaki H, Gouchi A, Morimoto Y, Ariki N, Funaki M, Tanaka N. Autologous serum deprivation restored IL-1 receptor antagonist production by peripheral blood mononuclear cells in patients with gastric cancer. The Journal of international medical research 2000; 28(6): 277-287. [DOI:10.1177/147323000002800604]
10. Makino T, Noguchi Y, Yoshikawa T, Doi C, Nomura K. Circulating interleukin 6 concentrations and insulin resistance in patients with cancer. The British journal of surgery 1998; 85(12): 1658-62. [DOI:10.1046/j.1365-2168.1998.00938.x]
11. Ohno M, Kato M, Nakamura T, Saitoh Y. Gene expression for tumor necrosis factor alpha and its production in gastric cancer patients. Japanese journal of cancer research 1994; 85(10): 1029-1034. [DOI:10.1111/j.1349-7006.1994.tb02901.x]
12. C W Wu, C W Chi, M C Hsieh, M F Chao, W Y Lui FKP. Serum tumor necrosis factor in patients with gastric cancer. Anticancer research 1998; 18 (3A): 1597-1599.
13. M Krönke , G Hensel, C Schlüter, P Scheurich, S Schütze KP. Tumor necrosis factor and lymphotoxin gene expression in human tumor cell lines. Cancer research 1998; 48(19): 5417-5421.
14. Balkwill F, Burke F, Talbot D, Tavernier J, Osborne R, Naylor S, Talbot D, Durbin H, Tavernier J, Fiers W. Evidence for tumour necrosis factor/cachectin production in cancer. Lancet (London, England) 1987; 2(8570): 1229-1232. [DOI:10.1016/S0140-6736(87)91850-2]
15. Sánchez-Zauco N, Torres J, Gómez A, Camorlinga-Ponce M, Muñoz-Pérez L, Herrera-Goepfert R, Medrano-Guzmán R, Giono-Cerezo S, Maldonado-Bernal C. Circulating blood levels of IL-6, IFN-γ, and IL-10 as potential diagnostic biomarkers in gastric cancer: a controlled study. BMC Cancer 2017; 17(1): 384. [DOI:10.1186/s12885-017-3310-9]
16. Li J, Xu L, Run ZC, Feng W, Liu W, Zhang PJ, Li Z. Multiple cytokine profiling in serum for early detection of gastric cancer. World journal of gastroenterology 2018; 24(21): 2269-2278. [DOI:10.3748/wjg.v24.i21.2269]
17. Simó R, Barbosa-Desongles A, Lecube A, Hernandez C, Selva DM. Potential role of tumor necrosis factor-α in downregulating sex hormone-binding globulin. Diabetes 2012; 61(2): 372-382. [DOI:10.2337/db11-0727]
18. Wherry EJ, Kurachi M. Molecular and cellular insights into T cell exhaustion. Nature reviews. Immunology 2015; 15(8): 486-499. [DOI:10.1038/nri3862]
19. Zhao M, Kiernan CH, Stairiker CJ, Hope JL, Leon LG, van Meurs M, Brouwers-Haspels I, Boers R , Boers J , Gribnau J, van IJcken WFJ, Bindels EW, Hoogenboezem RM , Erkeland SJ, Mueller YM, Katsikis PD. Rapid in vitro generation of bona fide exhausted CD8+ T cells is accompanied by Tcf7 promotor methylation. PLoS pathogens 2020; 16(6). e1008555. [DOI:10.1371/journal.ppat.1008555]
20. Fu K, Hui B, Wang Q, Lu C, Shi W, Zhang Z, Rong D , Zhang B, Tian Z, Tang W, Cao H, Wang X, Chen Z. Single-cell RNA sequencing of immune cells in gastric cancer patients. Aging 2020; 12(3): 2747-2763. [DOI:10.18632/aging.102774]
21. Josephs SF, Ichim TE, Prince SM, Kesari S, Marincola FM, Escobedo AR, Jafri A. Unleashing endogenous TNF-alpha as a cancer immunotherapeutic. Journal of translational medicine 2018; 16(1): 242. [DOI:10.1186/s12967-018-1611-7]
22. Jiang H, Yu D, Yang P, Guo R, Kong M, Gao Y, Yu X , Lu X, Fan X. Revealing the transcriptional heterogeneity of organ-specific metastasis in human gastric cancer using single-cell RNA Sequencing. Clinical and translational medicine 2022; 12(2): e730. [DOI:10.1002/ctm2.730]
23. Jayasooriya RGPT, Moon DO, Park SR, Choi YH, Asami Y, Kim MO, Jang JH, Yeon Kim B, Seog Ahn J, Kim GY. Combined treatment with verrucarin A and tumor necrosis factor-α sensitizes apoptosis by overexpression of nuclear factor-kappaB-mediated Fas. Environmental toxicology and pharmacology 2013; 36(2): 303-310. [DOI:10.1016/j.etap.2013.04.008]
24. Fernandes J, Michel V, Camorlinga-Ponce M, Gomez A, Maldonado C, De Reuse H, Torres J, Touati E. Circulating mitochondrial DNA level, a noninvasive biomarker for the early detection of gastric cancer. Cancer epidemiology, biomarkers and prevention 2014; 23(11): 2430-2438. [DOI:10.1158/1055-9965.EPI-14-0471]
25. Konturek SJ, Starzynska T, Konturek PC, Karczewska E, Marlicz K, Lawniczak M, Jaroszewicz-Heigelman H, Bielanski W, Hartwich A, Ziemniak A, Hahn E G. Helicobacter pylori and CagA status, serum gastrin, interleukin-8 and gastric acid secretion in gastric cancer. Scandinavian journal of gastroenterology 2002; 37(8): 891-898. [DOI:10.1080/003655202760230838]

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