Volume 25, Issue 6 (11-2021)                   IBJ 2021, 25(6): 426-433 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ghaffari H, Tavakoli A, Faranoush M, Naderi A, Kiani S J, Sadeghipour A, et al . Molecular Investigation of Human Cytomegalovirus and Epstein-Barr virus in Glioblastoma Brain Tumor: A Case-Control Study in Iran. IBJ 2021; 25 (6) :426-433
URL: http://ibj.pasteur.ac.ir/article-1-3156-en.html
Molecular Investigation of Human Cytomegalovirus and Epstein-Barr virus in Glioblastoma Brain Tumor: A Case-Control Study in Iran
Hadi Ghaffari , Ahmad Tavakoli , Mohammad Faranoush , Ali Naderi , Seyed Jalal Kiani , Alireza Sadeghipour , Davod Javanmard , Mohammad Farahmand , Saied Ghorbani , Farnoush Sedaghati , Seyed Hamidreza Monavari
Abstract:  
Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unclear etiology. Our study aimed to investigate the molecular prevalence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) infections in patients with glioblastoma multiforme (GBM). Methods: This case-control study was conducted on 42 FFPE brain tumor samples from GBM patients and 42 brain autopsies from subjects without neurological disorders. The presence of EBV and HCMV DNA was determined, using PCR and nested-PCR assays, respectively. Results: HCMV DNA was detected in 3 out of 42 (7.1%) of GBM samples and was absent from the control group (p = 0.07). Importantly, EBV DNA was detected in 9 out of 42 (21.4%) brain tissue specimens of GBM subjects, but again in none of the control group (p = 0.001). Conclusion: Our findings indicate that infection with EBV is associated with GBM.
Keywords: Brain tumor, Epstein-Barr virus, Glioblastoma, Human cytomegalovirus
Full-Text [PDF 704 kb]      
Type of Study: Full Length/Original Article | Subject: Molecular Microbiology
References
1. Hanif F, Muzaffar K, Perveen K, Malhi SM, Simjee SU. Glioblastoma multiforme: a review of its epidemiology and pathogenesis through clinical presentation and treatment. Asian pacific journal of cancer prevention 2017; 18(1): 3-9.
2. Paolillo M, Boselli C, Schinelli S. Glioblastoma under siege: an overview of current therapeutic strategies. Brain sciences 2018; 8(1): 15. [DOI:10.3390/brainsci8010015]
3. McFaline-Figueroa JR, Wen PY. The viral connection to glioblastoma. Current infectious disease reports 2017; 19(2): 5. [DOI:10.1007/s11908-017-0563-z]
4. Bokharaei-Salim F, Keyvani H, Monavari SH, Alavian SM, Fakhim S, Nasseri S. Distribution of hepatitis C virus genotypes among Azerbaijani patients in capital city of Iran-Tehran. Hepatitis monthly 2013; 13(9): e13699. [DOI:10.5812/hepatmon.13699]
5. Sadeghi F, Salehi-Vaziri M, Ghodsi SM, Alizadeh A, Bokharaei-Salim F, Saroukalaei ST, Mirblouki M, Monavari H, Keyvani H. Prevalence of JC polyomavirus large T antigen sequences among Iranian patients with central nervous system tumors. Archives of virology 2015; 160(1): 61-68. [DOI:10.1007/s00705-014-2230-0]
6. Salehi-Vaziri M, Sadeghi F, Alamsi-Hashiani A, Haeri H, Monavari SH, Keyvani H. Merkel cell polyomavirus and human papillomavirus infections in cervical disease in Iranian women. Archives of virology 2015; 160(5): 1181-1187. [DOI:10.1007/s00705-015-2368-4]
7. Salehi-Vaziri M, Sadeghi F, Bokharaei-Salim F, Younesi S, Alinaghi S, Monavari SH, Keivani H. The prevalence and genotype distribution of human papillomavirus in the genital tract of males in Iran. Jundishapur journal of microbiology 2015; 8(12): e21912. [DOI:10.5812/jjm.21912]
8. Ludlow M, Kortekaas J, Herden C, Hoffmann B, Tappe D, Trebst C, Griffin D, Brindle H, Solomon T, Brown AS, Riel DV, Wolthers KC, Pajkrt D, Wohlsein P, Martina BE, Baumgärtner W, Verjans GM, Osterhaus A. Neurotropic virus infections as the cause of immediate and delayed neuropathology. Acta neuropathologica 2016; 131(2): 159-184. [DOI:10.1007/s00401-015-1511-3]
9. Joseph GP, McDermott R, Baryshnikova MA, Cobbs CS, Ulasov IV. Cytomegalovirus as an oncomodulatory agent in the progression of glioma. Cancer letters 2017; 384: 79-85. [DOI:10.1016/j.canlet.2016.10.022]
10. Akhtar S, Vranic S, Cyprian FS, Al Moustafa A-E. Epstein-Barr virus in gliomas: cause, association, or artifact? Frontiers in oncology 2018; 8: 123. [DOI:10.3389/fonc.2018.00123]
11. Shamran HA, Kadhim HS, Hussain AR, Kareem A, Taub DD, Price RL, Nagarkatti M, Nagarkatti PS, Singh UP. Detection of human cytomegalovirus in different histopathological types of glioma in Iraqi patients. Biomed research international 2015; 2015: 642652. [DOI:10.1155/2015/642652]
12. Nijman J, Mandemaker FS, Verboon-Maciolek MA, Aitken SC, van Loon AM, de Vries LS, Schuurman R. Genotype distribution, viral load and clinical characteristics of infants with postnatal or congenital cytomegalovirus infection. PLoS one 2014; 9(9): e108018. [DOI:10.1371/journal.pone.0108018]
13. Ai J, Xie Z, Liu C, Huang Z, Xu J. Analysis of EBNA-1 and LMP-1 variants in diseases associated with EBV infection in Chinese children. Virology journal 2012; 9(1): 1-8. [DOI:10.1186/1743-422X-9-13]
14. Rajesh Y, Pal I, Banik P, Chakraborty S, Borkar SA, Dey G, Mukherjee A, Mandal M. Insights into molecular therapy of glioma: current challenges and next generation blueprint. Acta pharmacologica sinica 2017; 38(5): 591-613. [DOI:10.1038/aps.2016.167]
15. Kofman A, Marcinkiewicz L, Dupart E, Lyshchev A, Martynov B, Ryndin A, Kotelevskaya E, Brown J, Schiff D, Abounader R. The roles of viruses in brain tumor initiation and oncomodulation. Journal of neuro-oncology 2011; 105(3): 451-466. [DOI:10.1007/s11060-011-0658-6]
16. Mitchell DA, Xie W, Schmittling R, Learn C, Friedman A, McLendon RE, Sampson JH. Sensitive detection of human cytomegalovirus in tumors and peripheral blood of patients diagnosed with glioblastoma. Neuro-oncology 2008; 10(1): 10-18. [DOI:10.1215/15228517-2007-035]
17. Sabatier J, Uro-Coste E, Pommepuy I, Labrousse F, Allart S, Tremoulet M, Delisle MB, Brousset P. Detection of human cytomegalovirus genome and gene products in central nervous system tumors. British journal of cancer 2005; 92(4): 747-750. [DOI:10.1038/sj.bjc.6602339]
18. Poltermann S, Schlehofer B, Steindorf K, Schnitzler P, Geletneky K, Schlehofer J. Lack of association of herpesviruses with brain tumors. Journal of neurovirology 2006; 12(2): 90-99. [DOI:10.1080/13550280600654573]
19. Santos CFd, Sakai VT, Machado MAdAM, Schippers DN, Greene AS. Reverse transcription and polymerase chain reaction: principles and applications in dentistry. Journal of applied oral science 2004; 12: 1-11. [DOI:10.1590/S1678-77572004000100002]
20. Denkert C, Loibl S, Kronenwett R, Budczies J, Von Törne C, Nekljudova V, Darb-Esfahani S, Solbach C, Sinn BV, Petry B, Müller BM, Hilfrich J, Altmann G, Staebler A, Roth C, Ataseven B, Kirchner T, Dietel T, Untch M, Minckwitz G. RNA-based determination of ESR1 and HER2 expression and response to neoadjuvant chemotherapy. Annals of oncology 2013; 24(3): 632-639. [DOI:10.1093/annonc/mds339]
21. Fonseca RF, Rosas SLB, Oliveira JA, Teixeira A, Alves G, Carvalho MdGC. Frequency of Epstein-Barr virus DNA sequences in human gliomas. Sao Paulo medical journal 2015; 133(1): 51-54. [DOI:10.1590/1516-3180.2013.1912814]
22. Alifieris C, Trafalis DT. Glioblastoma multiforme: Pathogenesis and treatment. Pharmacology and therapeutics 2015; 152: 63-82. [DOI:10.1016/j.pharmthera.2015.05.005]
23. Pandya J, Walling DM. Oncogenic activity of Epstein-Barr virus latent membrane protein 1 (LMP-1) is down-regulated by lytic LMP-1. Journal of virology 2006; 80(16): 8038-8046. [DOI:10.1128/JVI.00180-06]
24. Wrensch M, Weinberg A, Wiencke J, Miike R, Barger G, Kelsey K. Prevalence of antibodies to four herpesviruses among adults with glioma and controls. American journal of epidemiology 2001; 154(2): 161-165. [DOI:10.1093/aje/154.2.161]
25. Strong MJ, Blanchard E, Lin Z, Morris CA, Baddoo M, Taylor CM, Ware ML, Flemington EK. A comprehensive next generation sequencing-based virome assessment in brain tissue suggests no major virus-tumor association. Acta neuropathologica communications 2016; 4(1): 1-10. [DOI:10.1186/s40478-016-0338-z]
26. Loit MP, Adle-Biassette H, Bouazza S, Mazeron M-C, Manivet P, Lehmann-Che J, Teissier N, Mandonnet E, Michel Molina J. Multimodal techniques failed to detect cytomegalovirus in human glioblastoma samples. Journal of neurovirology 2019; 25(1): 50-56. [DOI:10.1007/s13365-018-0683-8]
27. Zavala-Vega S, Castro-Escarpulli G, Hernández-Santos H, Salinas-Lara C, Palma I, Mejía-Aranguré JM, Gelista-Herrera N, Rembao-Bojorquez D, Ochoa SA, Cruz-Córdova A, Xicohtencatl-Cortes J, Uribe-Gutiérrez G, Arellano-Galindo J. An overview of the infection of CMV, HSV 1/2 and EBV in Mexican patients with glioblastoma multiforme. Pathology, research and practice 2017; 213(3): 271-276. [DOI:10.1016/j.prp.2016.12.006]
28. Zhang P, Lehmann BD, Shyr Y, Guo Y. The utilization of formalin fixed-paraffin-embedded specimens in high throughput genomic studies. International journal of genomics 2017; 2017: 1926304. [DOI:10.1155/2017/1926304]
29. Smatti MK, Al-Sadeq DW, Ali NH, Pintus G, Abou-Saleh H, Nasrallah GK. Epstein-Barr virus epidemiology, serology, and genetic variability of LMP-1 oncogene among healthy population: an update. Frontiers in oncology 2018; 8: 211. [DOI:10.3389/fonc.2018.00211]
30. See HS, Yap YY, Yip WK, Seow HF. Epstein-Barr virus latent membrane protein-1 (LMP-1) 30-bp deletion and Xho I-loss is associated with type III nasopharyngeal carcinoma in Malaysia. World journal of surgical oncology 2008; 6(1): 1-10. [DOI:10.1186/1477-7819-6-18]
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Biomedical Journal

Designed & Developed by : Yektaweb