Volume 25, Issue 6 (11-2021)                   IBJ 2021, 25(6): 426-433 | Back to browse issues page

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Ghaffari H, Tavakoli A, Faranoush M, Naderi A, Kiani S J, Sadeghipour A, et al . Molecular Investigation of Human Cytomegalovirus and Epstein-Barr virus in Glioblastoma Brain Tumor: A Case-Control Study in Iran. IBJ 2021; 25 (6) :426-433
URL: http://ibj.pasteur.ac.ir/article-1-3156-en.html
Molecular Investigation of Human Cytomegalovirus and Epstein-Barr virus in Glioblastoma Brain Tumor: A Case-Control Study in Iran
Hadi Ghaffari , Ahmad Tavakoli , Mohammad Faranoush , Ali Naderi , Seyed Jalal Kiani , Alireza Sadeghipour , Davod Javanmard , Mohammad Farahmand , Saied Ghorbani , Farnoush Sedaghati , Seyed Hamidreza Monavari *
Abstract:  
Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unclear etiology. Our study aimed to investigate the molecular prevalence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) infections in patients with glioblastoma multiforme (GBM). Methods: This case-control study was conducted on 42 FFPE brain tumor samples from GBM patients and 42 brain autopsies from subjects without neurological disorders. The presence of EBV and HCMV DNA was determined, using PCR and nested-PCR assays, respectively. Results: HCMV DNA was detected in 3 out of 42 (7.1%) of GBM samples and was absent from the control group (p = 0.07). Importantly, EBV DNA was detected in 9 out of 42 (21.4%) brain tissue specimens of GBM subjects, but again in none of the control group (p = 0.001). Conclusion: Our findings indicate that infection with EBV is associated with GBM.
Keywords: Brain tumor, Epstein-Barr virus, Glioblastoma, Human cytomegalovirus
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Type of Study: Full Length/Original Article | Subject: Molecular Microbiology
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