:: Volume 21, Issue 6 (11-2017) ::
IBJ 2017, 21(6): 411-416 Back to browse issues page
E6-Specific Detection and Typing of Human Papillomaviruses in Oral Cavity Specimens from Iranian Patients
Hadi Razavi Nikoo , Abdollah Ardebili , Mehrdad Ravanshad , Fatemeh Rezaei , Ali Teimoori , Sayyad Khanizadeh , Mohammad Hassan Pouriayevali , Mehdi Ajorloo

Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36%) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55%), and 8 specimens were positive for HPV-11 (44.44%). Of the 18 infected specimens, 6 (33.32%) and 12 (66.65%) were identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies.

Keywords: Real-time PCR, Genotyping, Iran
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Type of Study: Short Communication | Subject: Molecular Microbiology
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DOI: 10.18869/acadpub.ibj.21.6.411
PMID: 28460428
PMCID: PMC5572438

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Razavi Nikoo H, Ardebili A, Ravanshad M, Rezaei F, Teimoori A, Khanizadeh S, et al . E6-Specific Detection and Typing of Human Papillomaviruses in Oral Cavity Specimens from Iranian Patients . IBJ. 2017; 21 (6) :411-416
URL: http://ibj.pasteur.ac.ir/article-1-2031-en.html
Volume 21, Issue 6 (11-2017) Back to browse issues page
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