Volume 21, Issue 6 (11-2017)                   ibj 2017, 21(6): 411-416 | Back to browse issues page


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Razavi Nikoo H, Ardebili A, Ravanshad M, Rezaei F, Teimoori A, Khanizadeh S, et al . E6-Specific Detection and Typing of Human Papillomaviruses in Oral Cavity Specimens from Iranian Patients . ibj. 2017; 21 (6) :411-416
URL: http://ibj.pasteur.ac.ir/article-1-2031-en.html
Abstract:  

Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36%) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55%), and 8 specimens were positive for HPV-11 (44.44%). Of the 18 infected specimens, 6 (33.32%) and 12 (66.65%) were identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies.

Type of Study: Short Communication | Subject: Molecular Microbiology

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