Volume 2, Issue 2 (3-1998)                   ibj 1998, 2(2): 79-82 | Back to browse issues page

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Bouzari S, Rechinsky V O. Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector . ibj. 1998; 2 (2) :79-82
URL: http://ibj.pasteur.ac.ir/article-1-870-en.html
DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with poly‌ethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active.
Type of Study: Full Length |

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