Iranian
Biomedical Journal
Volume 3, Issue 3 And 4 (7-1999)
IBJ 1999, 3(3 And 4): 89-91 |
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Kamali Sarvestani E, Khezri A, Vessal M, Ghaderi A. A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen. IBJ 1999; 3 (3 and 4) :89-91
URL: http://ibj.pasteur.ac.ir/article-1-838-en.html
URL: http://ibj.pasteur.ac.ir/article-1-838-en.html
Abstract:
Prostate-specific antigen (PSA) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a CM-Sephadex C-50 and by gel filtration on a Sephacryl S-200 column. A single 33-kDa protein band appeared in SDS-PAGE. High pressure liquid chromatography (HPLC) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five different isoforms of this protein. The immunoreactivity of the purified PSA was checked by Western blotting. This simple two-step method can be used for a large-scale preparation of the purified PSA for the clinical tests and also for further investigative studies on the biological properties of this protein.
Type of Study: Full Length/Original Article |
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