Volume 4, Issue 2 And 3 (3-2000)                   IBJ 2000, 4(2 And 3): 75-78 | Back to browse issues page

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Rezaee A, Behzadian Nejad Q, Kebriaeezadeh A. Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography. IBJ 2000; 4 (2 and 3) :75-78
URL: http://ibj.pasteur.ac.ir/article-1-825-en.html
A sensitive high performance liquid chromathography (HPLC) analytical procedure was developed for the quantitive determination of trimethoprim (TM* and sulphamethoxazole (SM) in commercial dosage forms. C18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate as aqueous phase and acetonitrile with 0.4% triethylamine as organic phase. The drugs were quantified at flow-rate of 1.2 ml/min, with ultraviolet detection at 260 nm. The minimum detectable quantities in assays were 100 ng/ml for SM and 75 ng/ml for TM. The method is well suited to routine application and adequate sensitivity with precision.
Type of Study: Full Length |

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