Iranian
Biomedical Journal
Volume 30, Issue 2 (Supplementary 2026)
IBJ 2026, 30(2): 6-6 |
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Mohammadali M, Irani S, Atyabi S M, Sharifi F. Cold Atmospheric Plasma Surface Modification of PCL/CMC Scaffold for Cartilage Tissue Engineering. IBJ 2026; 30 (2) :6-6
URL: http://ibj.pasteur.ac.ir/article-1-5494-en.html
URL: http://ibj.pasteur.ac.ir/article-1-5494-en.html
Abstract:
Introduction: Surface modification is a crucial aspect in tissue engineering (TE) applications. This study aimed to use cold atmospheric plasma (CAP) for the surface modification of polycaprolactone (PCL) and carboxymethyl chitosan (CMC), creating a practical scaffold for cartilage TE.
Materials and Methods: The morphology and chemical properties of the PCL/CMC scaffolds were evaluated by scanning electron microscopy, contact angle measurements, and Fourier-transform infrared spectroscopy. The biocompatibility of the treated scaffold was assayed on human mesenchymal stem cells (hMSCs) using MTT at 72 hours. The chondrogenic differentiation of hMSCs was studied using SOX9 and COL2 gene expression through polymerase chain reaction and by detecting COL2 protein using immunocytochemistry tests.
Results and Discussion: Electrospinning of PCL/CMC produced a structure resembling the natural extracellular matrix. CAP treatment improved the surface properties of PCL/CMC, introducing functional groups. Based on the in vitro results, PCL/CMC was biocompatible, and specific genes related to chondrogenic differentiation, SOX9 and COL2, were expressed. In addition, the detection of chondrogenic differentiation of hMSCs was confirmed by COL2 protein.
Conclusion: PCL polymer exhibits hydrophobic characteristics and has a limited capacity for cellular recognition. The combination of PCL and CMC effectively addresses these limitations. Thus, plasma surface modification could enhance the overall performance of the scaffold.
Materials and Methods: The morphology and chemical properties of the PCL/CMC scaffolds were evaluated by scanning electron microscopy, contact angle measurements, and Fourier-transform infrared spectroscopy. The biocompatibility of the treated scaffold was assayed on human mesenchymal stem cells (hMSCs) using MTT at 72 hours. The chondrogenic differentiation of hMSCs was studied using SOX9 and COL2 gene expression through polymerase chain reaction and by detecting COL2 protein using immunocytochemistry tests.
Results and Discussion: Electrospinning of PCL/CMC produced a structure resembling the natural extracellular matrix. CAP treatment improved the surface properties of PCL/CMC, introducing functional groups. Based on the in vitro results, PCL/CMC was biocompatible, and specific genes related to chondrogenic differentiation, SOX9 and COL2, were expressed. In addition, the detection of chondrogenic differentiation of hMSCs was confirmed by COL2 protein.
Conclusion: PCL polymer exhibits hydrophobic characteristics and has a limited capacity for cellular recognition. The combination of PCL and CMC effectively addresses these limitations. Thus, plasma surface modification could enhance the overall performance of the scaffold.
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Keywords: Chondrogenic differentiation, Cold atmospheric plasma, Surface modification, Tissue engineering
Type of Study: Congress |
Subject:
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