Iranian
Biomedical Journal
Volume 30, Issue 1 (1-2026)
IBJ 2026, 30(1): 91-97 |
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Eskandari T, Shams M, Khanari A, Kahrizi A, Asgarian-Omran H, Valadan R, et al . Effect of HIF-1α and LDHA Blockade on Gene Expression of Tumor Immune Evasion in Myeloid Leukemia Cell Lines. IBJ 2026; 30 (1) :91-97
URL: http://ibj.pasteur.ac.ir/article-1-5324-en.html
URL: http://ibj.pasteur.ac.ir/article-1-5324-en.html
Tamana Eskandari 
, Mehrshad Shams , Ali Khanari , Amir Kahrizi , Hossein Asgarian-Omran , Reza Valadan , Mohsen Tehrani , Saeid Taghiloo *
, Mehrshad Shams , Ali Khanari , Amir Kahrizi , Hossein Asgarian-Omran , Reza Valadan , Mohsen Tehrani , Saeid Taghiloo *
Abstract:
Background: Tumor cells utilize metabolic reprogramming to obtain the energy required for rapid proliferation. In this study, we blocked two key factors, hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDHA), in the metabolic pathway in acute myeloid leukemia (AML) cell lines to evaluate their effect on the expression of genes involved in tumor evasion.
Methods: Silibinin and sodium oxamate, as HIF-1α and LDHA blockers, respectively, were used to treat K-562 and HL-60 cells. The MTT assay was used to assess cell viability, and quantitative reverse transcription PCR was used to measure the mRNA expression levels of PD-L1, CD47, and CD155.
Results: Both K-562 and HL-60 cells showed a significant decrease in CD47 expression. Additionally, HL-60 cells indicated a significant suppression of CD155 expression. In contrast, PD-L1 expression remained unchanged after treatment.
Conclusion: Our findings suggest that blocking signaling pathways involved in metabolic reprogramming of cancer cells could be a promising approach for modulating the expression of certain immune checkpoint ligands, warranting further investigation.
Methods: Silibinin and sodium oxamate, as HIF-1α and LDHA blockers, respectively, were used to treat K-562 and HL-60 cells. The MTT assay was used to assess cell viability, and quantitative reverse transcription PCR was used to measure the mRNA expression levels of PD-L1, CD47, and CD155.
Results: Both K-562 and HL-60 cells showed a significant decrease in CD47 expression. Additionally, HL-60 cells indicated a significant suppression of CD155 expression. In contrast, PD-L1 expression remained unchanged after treatment.
Conclusion: Our findings suggest that blocking signaling pathways involved in metabolic reprogramming of cancer cells could be a promising approach for modulating the expression of certain immune checkpoint ligands, warranting further investigation.
Type of Study: Full Length/Original Article |
Subject:
Cancer Biology
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