Iranian
Biomedical Journal
Abstract:
Background: Prolactin is a neurohypophyseal hormone that plays a major role in behavior, growth, and angiogenesis. CAB suppresses the effects of PRL by targeting the D2R. In this study, we used in silico and in vivo approaches to examine the side effects of CAB using chicken as a model.
Methods: Thirty fertilized eggs were equally divided into three groups: one control and two experimental groups treated with CAB at doses of 0.5 mg/kg and 1 mg/kg, respectively. After 18 days of experiment, embryo weight, average body length, chorioallantoic vessels length, and relative PRL gene expression were analyzed. Also, prolactin-protein interaction and functional pathways regulated by PRL and associated proteins were investigated using the STRING database.
Results: Macroscopic analysis showed a significant increase (17.54 ± 0.28) and decrease (12.92 ± 0.38) in weight in the 0.5 mg/kg and 1 mg/kg groups, respectively, as compared to the control. A significant reduction in body size and an increase in chorionic vessel development were observed only in the 0.5 mg/kg group. PRL expression levels significantly decreased in both CAB-treated groups. In silico analysis revealed PRL interactions with INS and POMC proteins, which are conserved between H. sapiens and G. gallus.
Conclusion: Our findings indicate that CAB not only reduces prolactin levels but also affects fetal weight and body size.
Methods: Thirty fertilized eggs were equally divided into three groups: one control and two experimental groups treated with CAB at doses of 0.5 mg/kg and 1 mg/kg, respectively. After 18 days of experiment, embryo weight, average body length, chorioallantoic vessels length, and relative PRL gene expression were analyzed. Also, prolactin-protein interaction and functional pathways regulated by PRL and associated proteins were investigated using the STRING database.
Results: Macroscopic analysis showed a significant increase (17.54 ± 0.28) and decrease (12.92 ± 0.38) in weight in the 0.5 mg/kg and 1 mg/kg groups, respectively, as compared to the control. A significant reduction in body size and an increase in chorionic vessel development were observed only in the 0.5 mg/kg group. PRL expression levels significantly decreased in both CAB-treated groups. In silico analysis revealed PRL interactions with INS and POMC proteins, which are conserved between H. sapiens and G. gallus.
Conclusion: Our findings indicate that CAB not only reduces prolactin levels but also affects fetal weight and body size.
Type of Study: Full Length/Original Article |
Subject:
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