Iranian
Biomedical Journal
Volume 28, Issue 5 And 6 (9-2024)
IBJ 2024, 28(5 And 6): 265-272 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mpho Khala M, van der Spoel van Dijk A, Hildah Mfengwana P. Anti-Inflammatory, Cytotoxic, and Anti-Tuberculosis Properties of Selected Fabaceae Medicinal Plants through in vitro Studies. IBJ 2024; 28 (5 and 6) :265-272
URL: http://ibj.pasteur.ac.ir/article-1-4356-en.html
URL: http://ibj.pasteur.ac.ir/article-1-4356-en.html
Abstract:
Background: Tuberculosis (TB) is among the top ten causes of death in South Africa and worldwide and remains a high priority of the World Health Organization. South Africa has a rich tradition for using medicinal plants to treat various diseases, including TB; however, the safety and efficacy of these plants require thorough investigation and confirmation. This study examined the anti-inflammatory and anti-TB activities of three indigenous medicinal plants against two strains of TB.
Methods: Water and methanol root extracts of E. elephantina (Burch.), L. lanceolata, and T. burchellianum were tested in vitro for their activity against Latin American Mediterranean (LAM) and East African Indian (EAI) lineages. Additionally, the phytochemical screening, anti-inflammatory properties, and cytotoxicity activities of these extracts were evaluated.
Results: Aqueous extracts of E. elephantina and T. burchellianum exhibited anti-inflammatory activity at a concentration of 200 μg/ml, while
L. lanceolata demonstrated no activity on macrophage cells. The
E. elephantina extracts showed no cytotoxicity against the Vero cells at high concentration (200 μg/ml); however, both L. lanceolata and T. burchellianum aqueous extracts were found to be cytotoxic at 200 μg/ml. Unfortunately, none of the three plant extracts showed anti-TB activity against LAM and EAI stains.
Conclusion: While the tested extracts lacked direct anti-TB effects, their anti-inflammatory properties and safety profile highlight their potential for adjunctive therapy in TB management or other inflammatory conditions. Further studies are needed to explore the mechanisms and potential clinical applications of these findings.
Methods: Water and methanol root extracts of E. elephantina (Burch.), L. lanceolata, and T. burchellianum were tested in vitro for their activity against Latin American Mediterranean (LAM) and East African Indian (EAI) lineages. Additionally, the phytochemical screening, anti-inflammatory properties, and cytotoxicity activities of these extracts were evaluated.
Results: Aqueous extracts of E. elephantina and T. burchellianum exhibited anti-inflammatory activity at a concentration of 200 μg/ml, while
L. lanceolata demonstrated no activity on macrophage cells. The
E. elephantina extracts showed no cytotoxicity against the Vero cells at high concentration (200 μg/ml); however, both L. lanceolata and T. burchellianum aqueous extracts were found to be cytotoxic at 200 μg/ml. Unfortunately, none of the three plant extracts showed anti-TB activity against LAM and EAI stains.
Conclusion: While the tested extracts lacked direct anti-TB effects, their anti-inflammatory properties and safety profile highlight their potential for adjunctive therapy in TB management or other inflammatory conditions. Further studies are needed to explore the mechanisms and potential clinical applications of these findings.
Type of Study: Full Length/Original Article |
Subject:
Related Fields
References
1. Zumla A, Rao M, Parida SK, Keshavjee S, Cassell G, Wallis R, et al. Inflammation and tuberculosis: host-directed therapies. J Intern Med. 2015;277(4):373-87. [DOI:10.1111/joim.12256]
2. World Health Organization (WHO). Global Tuberculosis Report 2021. Available at: https://www.who.int/publications/i/item/9789240037021.
3. Long, R., Divangahi, M., & Schwartzman, K. Chapter 2: Transmission and pathogenesis of tuberculosis. Can J Respir Crit Care Sleep Med. 2022;6(sup1):22-32. [DOI:10.1080/24745332.2022.2035540]
4. Babalık A, Ulus IH, Bakirci N, Kuyucu T, Arpag H, Dagyildiz L, et al. Pharmacokinetics and serum concentrations of antimycobacterial drugs in adult Turkish patients. Int J Tuberc Lung Dis. 2013;17(11):1442-7. [DOI:10.5588/ijtld.12.0771]
5. Imam F, Sharma M, Khayyam KU, Al-Harbi NO, Rashid MK, Ali MD, et al. Adverse drug reaction prevalence and mechanisms of action of first-line anti-tubercular drugs. Saudi Pharm J. 2020;28(3):316-24. [DOI:10.1016/j.jsps.2020.01.011]
6. Ndhlala AR, Stafford GI, Finnie JF, Van Staden J. Commercial herbal preparations in KwaZulu-Natal, South Africa: The urban face of traditional medicine. S Afr J. 2011;77(4):830-43. [DOI:10.1016/j.sajb.2011.09.002]
7. Mushtaq S, Abbasi BH, Uzair B, Abbasi R. Natural products as reservoirs of novel therapeutic agents. EXCLI J. 2018;4(17):420-51.
8. Maroyi A. Elephantorrhiza elephantina: Traditional uses, phytochemistry, and pharmacology of an important medicinal plant species in Southern Africa. Evid-Based Complementary Altern Med. 2017:6403905. [DOI:10.1155/2017/6403905]
9. Mpofu SJ, Msagati TA, Krause RW. Cytotoxicity, phytochemical analysis and antioxidant activity of crude extracts from rhizomes of Elephantorrhiza elephantina and Pentanisia prunelloides. Afr J Tradit Complement Altern Med. 2013;11(1):34-52. [DOI:10.4314/ajtcam.v11i1.6]
10. Tlotlo R, Mfengwana PH, Olivier D. Review on literature of the plant Elephantorrhiza elephantine on its healing properties and recent acquired knowledge of its medicinal activities. Pharmacogn J. 2022;14(3):715-21. [DOI:10.5530/pj.2022.14.91]
11. Useful Temperate Plants. Leobordea Lanceolata. 2022 [updated 2024 October 13]. Available from: http://temperate.theferns.info/plant/Leobordea+lanceolata.
12. Kose LS, Moteetee A, Van Vuuren S. Ethnobotanical survey of medicinal plants used in the Maseru district of Lesotho. J Ethnopharmacol. 2015;170:184-200. [DOI:10.1016/j.jep.2015.04.047]
13. Ntsoelinyane PH, Mashele S. Phytochemical screening, antibacterial and antioxidant activities of Asparagus laricinus leaf and stem extracts. Bangladesh J Pharmacol. 2014; DOI:10.3329/bjp.v9i1.16967 . [DOI:10.3329/bjp.v9i1.16967]
14. Polo-Ma-Abiele M, Samson M, Idah TM. In vitro antibacterial, antioxidant and anti-Inflammatory effects of Senecio asperulus and Gunnera perpensa from Mohale's Hoek, Lesotho. Pharmacogn J. 2019; 11(4):730-9. [DOI:10.5530/pj.2019.11.116]
15. Mfengwana PH, Mashele SS, Manduna IT. Cytotoxicity and cell cycle analysis of Asparagus laricinus Burch. and Senecio asperulus DC. on breast and prostate cancer cell lines. Heliyon. 2019;5(5):e01666. [DOI:10.1016/j.heliyon.2019.e01666]
16. Siddiqi S, Rüsch-Gerdes S. For BACTECTM MGIT 960TM TB System (Also applicable for Manual MGIT) Mycobacteria Growth Indicator Tube (MGIT) Culture and Drug Susceptibility Demonstration Projects [Internet]. 2006. Available from: https://www.finddx.org/wp-content/uploads/2023/02/20061101_rep_mgit_manual_FV_EN.pdf
17. Muefong CN, Sutherland JS. Neutrophils in tuberculosis-associated inflammation and lung pathology. Front Immunol. 2020;11:962. [DOI:10.3389/fimmu.2020.00962]
18. Olaokun OO, Alaba AE, Ligege K, Mkolo NM. Phytochemical content, antidiabetes, anti-inflammatory antioxidant and cytotoxic activity of leaf extracts of Elephantorrhiza elephantina (Burch.) Skeels. S Afr J Bot. 2020;128:319-25. [DOI:10.1016/j.sajb.2019.11.030]
19. Kishore N, Mishra BB, Tripathi V, Tiwari VK. Alkaloids as potential anti-tubercular agents. Fitoterapia 2009;80(3):149-63. [DOI:10.1016/j.fitote.2009.01.002]
20. Ullrich SF, Rothauer A, Hagels H, Kayser O. Influence of light, temperature, and macronutrients on growth and scopolamine biosynthesis in Duboisia species. Planta Med. 2017;83(11):937-45. [DOI:10.1055/s-0043-106435]
21. Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang Q. Response of plant secondary metabolites to environmental factors. Molecules. 2018;23(4):762. [DOI:10.3390/molecules23040762]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
IBJ is a member of Committee on Publication Ethics (COPE)
and follows its guidelines |
The articles of this journal are licensed under
a Creative Commons Attribution-NonDerivatives 4.0 International License. .png) |
 IBJ is owned and published by Pasteur Institute of Iran |