Melittin as an Activator of the Autophagy and Unfolded Protein  Response Pathways in Colorectal HCT116 Cell Line

Zamani, Mozhdeh; Bozorg-Ghalati, Farzaneh; Mokarram, Pooneh

doi:10.61186/ibj.3993

Volume 28, Issue 1 (1-2024) IBJ 2024, 28(1): 46-52 | Back to browse issues page

‎ 10.61186/ibj.3993

‎ 20.1001.1.1028852.2024.28.1.11.6

PMID: 38445441

Ethics code: IR.SUMS.REC.1401.127

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Zamani M, Bozorg-Ghalati F, Mokarram P. Melittin as an Activator of the Autophagy and Unfolded Protein Response Pathways in Colorectal HCT116 Cell Line. IBJ 2024; 28 (1) :46-52
URL: http://ibj.pasteur.ac.ir/article-1-3993-en.html

Melittin as an Activator of the Autophagy and Unfolded Protein Response Pathways in Colorectal HCT116 Cell Line

Mozhdeh Zamani

, Farzaneh Bozorg-Ghalati

, Pooneh Mokarram ^*

Abstract:

Background: The surface properties of dental and orthopedic implants are directly related to their osseointegration rate. Coating and/or modifying the implant surface might reduce the time of healing. In this study, we aimed to examine the effects of a hybrid surface consisting of a brushite surface coating and cross-linked water-soluble eggshell membrane protein on the osseointegration of titanium (Ti) screws under in vivo conditions.
Methods: Twenty Ti alloy screws were implanted monocortically in anteromedial regions of New Zealand rabbit tibiae. Ten screws were untreated and used as controls. The remaining 10 screws were coated with calcium phosphate and following cross-linked with ostrich eggshell membrane protein. All rabbits were sacrificed six weeks after the surgery. Peri-screw tissues were evaluated by micro-computed tomography (µ-CT), histological and histomorphometrical methods.
Results: The μ-CT assessments indicated that the experimental group had significantly higher mean bone surface area (BSA) and trabeculae number (TbN) than those of the control group (p ˂ 0.05). Bone surface area (BV), trabecular separation (TbSp), trabecular thickness (TbTh), and bone mineral density (BMD) scores of the control and experimental groups were quite similar (p > 0.05). The vascularization score of the experimental group was significantly higher than the control group (4.29 vs. 0.92%). No sign of the graft-versus-host reaction was observed.
Conclusion: Our findings reveal that coating Ti alloy implants with calcium phosphate cross-linked with ostrich eggshell membrane protein increases the osseointegration of Ti alloy screws by increasing the bone surface area, number of trabeculae and vascularization in the implant site.

Keywords: Bee venom, Colorectal neoplasms, Unfolded protein response, Autophagy

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Type of Study: Full Length/Original Article | Subject: Cancer Biology

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