Iranian
Biomedical Journal
Volume 26, Issue 6 (11-2022)
IBJ 2022, 26(6): 475-484 |
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Zargan S, Salehi Barough M, Zargan J, Shayesteh M, Haji Noor Mohammadi A, Mousavi M et al . Combined Effect of Neutron Radiation and Curcumin on Breast Cancer Cells Cytotoxicity in 3D Culture Medium. IBJ 2022; 26 (6) :475-484
URL: http://ibj.pasteur.ac.ir/article-1-3756-en.html
URL: http://ibj.pasteur.ac.ir/article-1-3756-en.html
Sajedeh Zargan 
, Mahdi Salehi Barough * , Jamil Zargan , Mohsen Shayesteh , Ashkan Haji Noor Mohammadi , Mohsen Mousavi , Hani Keshavarz Alikhani
, Mahdi Salehi Barough * , Jamil Zargan , Mohsen Shayesteh , Ashkan Haji Noor Mohammadi , Mohsen Mousavi , Hani Keshavarz Alikhani
Abstract:
Introduction: Chemotherapy, biotherapy, and radiotherapy play a limited but important role in treating breast cancer. For more efficient treatment, combination therapy could be an appropriate option. In this study, radiotherapy using neutron radiation emitted from a 241Am-Be neutron source, as well as biotherapy using curcumin (80 μM) was combined to investigate the efficiency of treatment towards MCF-7 breast cancer in a three dimensional (3D) culture medium.
Methods: MTT, neutral red uptake assay, nitric oxide, glutathione assay, catalase, cytochrome c, comet assay, and caspase-3 were used to determine the effect of neutron radiation and also neutron and curcumin combination on the viability of cancer cells.
Results: The results of cytotoxicity test showed that neutron irradiation with or without curcumin at 5, 10, 15, and 20 h reduced the survival of tumor cells. Moreover, the rate of apoptosis due to the neutron effect at different irradiation times enhanced with the increasing time.
Conclusion: Due to the significant anticancer effect of curcumin in 3D culture, using this molecule before or after neutron therapy is recommended.
Methods: MTT, neutral red uptake assay, nitric oxide, glutathione assay, catalase, cytochrome c, comet assay, and caspase-3 were used to determine the effect of neutron radiation and also neutron and curcumin combination on the viability of cancer cells.
Results: The results of cytotoxicity test showed that neutron irradiation with or without curcumin at 5, 10, 15, and 20 h reduced the survival of tumor cells. Moreover, the rate of apoptosis due to the neutron effect at different irradiation times enhanced with the increasing time.
Conclusion: Due to the significant anticancer effect of curcumin in 3D culture, using this molecule before or after neutron therapy is recommended.
Type of Study: Full Length/Original Article |
Subject:
Cancer Biology
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