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Background: Lysozyme is a part of human and animal non-cellular immunity. The regulation of its activity by hormones is poorly studied. The aim of this study is to test the in vitro activity of lysozyme in the presence of catecholamines, natriuretic hormones and estradiol. Methods: The hormones were incubated with lysozyme and its activity was further determined using a test culture of Micrococcus luteus (in the early exponential growth stage). The activity was assessed by the rate of change in the optical density of the test culture. Molecular docking was performed using the online docking service (http://www.swissdock.ch/docking), and further analysis and visualization of molecular structures in the UCSF Chimera 1.15rc program. Results: A study showed that epinephrine and norepinephrine increased lysozyme activity by up to 180% compared to the hormone-free enzyme. Changing the pH of the medium from 6.3 to 5.5 increased the lysozyme activity in the presence of estradiol up to 150-200%. The results showed that exposure to hormones can modify lysozyme activity, and this effect depends on the temperature and pH.  The molecular docking revealed a decrease in the activation energy of the enzyme active center during the interaction of catecholamines with the amino acid residues asp52 and glu35 of the reaction center. Conclusion: Our study demonstrates an additional mechanism for involvement of lysozyme in humoral regulation of nonspecific immunity with respect to human pathogenic microflora and bacterial skin commensals - by direct modulation of its activity by human hormones.
Type of Study: Full Length | Subject: Enzymology and Protein Chemistry

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