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Abstract:  
Background: Tuberculosis (TB) infection still represents a global health issue affecting patients worldwide. Its control strategies may be not as effective as it should be, specifically in case of resistant strains of Mycobacterium tuberculosis (M.tb). The role of mycobacterial methyltransferases (MTases) in TB infection can be fundamental in this regard but has not been broadly deciphered. Methods: Five resistant isolates of M.tb were obtained from Mycobacteriology Department of Pasteur Institute of Iran. M.tb H37Rv (ATCC 27249) was used as a reference strain. Seven putative mycobacterial MTase genes (Rv0645c, Rv2966c, Rv1988, Rv1694, Rv3919c, Rv2756c, and Rv3263) and Rv1392 as S-adenosylmethionine (SAM) synthase were selected for analysis. PCR-sequencing and quantitative reverse transcription‑polymerase chain reaction (qRT-PCR) was performed to compare mutations and expression levels of MTases in different strains. The 2−ΔΔCt method was used for calculation of the relative expression levels of these genes. Results: Only two mutations have been reported in INH strain for Rv3919c (T to G in codon 341) and Rv1392 (G to A in codon 97) genes. Overexpression of Rv0645c, Rv2756c, Rv3263, and Rv2966c has been detected in all sensitive and resistant isolates. Whereas, Rv1988 and Rv3919c were decreased only in the sensitive strain and Rv1694 increased in the sensitive strain. Finally, the Rv1392 expression level has been decreased in INH isolate. Conclusion: We found links for correlation between mycobacterial MTases expression and resistance to antibiotics in M.tb strains. Undeniably, some MTases are virulence factor that specifically hijack the host defense mechanism. Further evaluations are needed to explore the complete impact of mycobacterial MTases within specific strains of M.tb to introduce novel diagnosis and treatment strategies.
     
Type of Study: Full Length | Subject: Molecular Microbiology

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