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Background: The presence of microbiome in the blood samples of healthy individuals has been addressed. However, no information can be found on the healthy human blood microbiome of Iranian subjects. The current study is thus aimed to investigate the existence of bacteria or bacterial DNA in healthy individuals. Methods: Blood samples of healthy subjects were incubated in BHI broth at 37 ̊C for 72 h. 16S rRNA PCR (Polymerase Chain Reaction) and sequencing were performed to analyze bacterial isolates. 16S rRNA PCR was directly carried out on DNA samples extracted from the blood of healthy individuals. Next-generation sequencing was conducted on blood samples with culture-positive results. Results: Fifty blood samples were tested, and six samples were positive by culture as confirmed by Gram staining and microscopy. The obtained 16S rRNA sequences of cultured bacterial isolates revealed the presence of Bacilli and Staphylococcus species by clustering in the GeneBank database (≥ 97% identity). The 16S rRNA gene sequencing results of one non-cultured blood specimen showed the presence of Bulkhulderia. Next-generation sequencing (NGS) results illustrated the presence of Romboutsia, Lactobacillus, Streptococcus, Bacteroides, and Staphylococcus in blood samples of positive cultures. Conclusion: The dormant blood microbiome of healthy individuals may give the idea that the steady transfer of bacteria into the blood, not necessarily leads to sepsis. However, the origins and identities of blood-associated bacterial rDNA sequences need more evaluation in the healthy population.
Type of Study: Full Length | Subject: Molecular Microbiology

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