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Mesenchymal stem cells (MSCs) enhance tissue repair through paracrine effects following transplantation, and one of the important factors contributing to the repair of damaged tissues is the versican protein. Using MSC conditioned medium for the culture of monocytes may increase versican protein production and could be a good alternative for transplantation of mesenchymal stem cells. In this study, the effect of culture medium conditioned by human mesenchymal stem cells on the expression of the versican gene in peripheral blood mononuclear cells (PBMC) in hypoxia-mimetic and normoxic conditions was investigated. The conditioned media used were derived from either adipose tissue or from Wharton jelly. Flow cytometry for surface markers (CD105, CD73 and CD90) was used to confirm mesenchymal stem cells. The peripheral blood mononuclear cells were isolated and cultured with the culture media of the MSC derived from either the adipose tissue or Wharton jelly. Desferrioxamine and cobalt chloride (200 µM and 300 µM final concentrations respectively) were added to monocytes media in order to induce hypoxia-mimetic conditions. Western blotting was used to detect HIF-1α protein and confirm hypoxia-mimetic conditions in PBMC. Versican gene expression was assessed in PBMC using Real Time-PCR. Western blotting showed that expression of HIF-1α in PBMC was significantly increased (P <0.01). RT-PCR results showed that expression of the versican and VEGF genes in PBMC increased significantly (P<0.01) in hypoxia-mimetic conditions as compared to normoxia. The present study showed that the secreted factors of MSCs can be replaced by direct use of MSCs to improve damaged tissues.


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