Volume 26, Issue 1 (1-2022)                   ibj 2022, 26(1): 53-63 | Back to browse issues page


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Mohammadi M, Salehzadeh A, Talesh Sasani S, Tarang A. The miR526b-5p-Related Single Nucleotide Polymorphisms, rs72618599, Located in 3'-UTR of TCF3 Gene, is Associated with the Risk of Breast and Gastric Cancers. ibj. 2022; 26 (1) :53-63
URL: http://ibj.pasteur.ac.ir/article-1-3379-en.html
Abstract:  
Introduction: Single nucleotide polymorphisms result in dysregulation of the proto-oncogene TCF3 gene, which is associated with the development, metastasis, and chemoresistance of different malignancies. Methods: GSE10810 microarray dataset and GEPIA2 online software were used to find differentially expressed genes and the TCF3 status in breast cancer (BC) and gastric cancer (GC), respectively. Plots and figures of microarray analysis were prepared by ggplot2 and pheatmap packages. Differentially expressed genes were obtained by the Bioconductor limma package. In silico analysis was used to predict the functions of rs72618599. BC (n = 123), GC (n = 132) and healthy age and gender matched controls (n = 184) were genotyped, using the high-resolution melting technique. Results: Based on the allelic comparison study, C allele of rs72618599 was associated with the BC tumor stage IV (66.1%, 78/120, p < 0.0001) and grade III (52.4%, 55/72, p < 0.0001), while the T allele was associated with metastasis (84.2%, 10/162, p < 0.0001). However, in GC patients, the C allele was significantly correlated with H. pylori infection (51.7%, 30/58, p = 0.008), stage III of primary tumors (47.7%, 62/88, p = 0.017), stage II of lymph node status (35.5%, 44/74, p = 0.017), and metastasis (52.9%, 90/132, p = 0.044). In silico analysis predicted that rs72618599 leads to the creation of a binding site for hsa-miR526b-5p in the 3′-UTR of TCF3 transcript. Conclusion: Regarding the rs72618599 SNP, the C allele, is associated with poor prognosis of BC and GC. Furthermore, rs72618599 may be associated with cancer progression by altering the regulatory affinity of hsa-miR526b-5p to 3′-UTR of TCF3.
Type of Study: Full Length | Subject: Molecular Genetics & Genomics

References
1. Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Piñeros M, Znaor A, Bray F. Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. International journal of cancer 2019; 144(8): 1941-53. [DOI:10.1002/ijc.31937]
2. Mirzaeyan P, Shokrzadeh M, Salehzadeh A, Ajamian F. Association of estrogen receptor 1 (ESR1) gene (rs2234693) polymorphism, ESR1 promoter methylation status, and serum heavy metals concentration, with breast cancer: A study on Iranian women population. Meta gene 2020; 26: 100802. [DOI:10.1016/j.mgene.2020.100802]
3. Salehiniya H, Haghighat S, Parsaeian M, Majdzadeh R, Mansournia M, Nedjat S. Iranian breast cancer risk assessment study (IRBCRAS): a case control study protocol. World cancer research journal 2018; 5: 1-5.
4. Sadjadi A, Nouraie M, Mohagheghi MA, Mousavi-Jarrahi A, Malekezadeh R, Parkin DM. Cancer occurrence in Iran in 2002, an international perspective. Asian pacific journalof cancer prevention 2005; 6(3): 359-63.
5. Ghaffari HR, Yunesian M, Nabizadeh N, Nasseri S, Sadjadi A, Pourfarzi F, Poustchi H, Eshraghian A. Environmental etiology of gastric cancer in Iran: a systematic review focusing on drinking water, soil, food, radiation, and geographical conditions. Environmental science and pollution research 2019; 26(11): 10487-10495. [DOI:10.1007/s11356-019-04493-8]
6. Amieva M, Peek RMJr. Pathobiology of Helicobacter pylori-induced gastric cancer. Gastroenterology 2016; 150(1): 64-78. [DOI:10.1053/j.gastro.2015.09.004]
7. Fan, Y, Gu X, Pan H, Dai Z, Zou C, Gao Z, Zhang H. Association of genetic polymorphisms in TNFRSF11 with the progression of genetic susceptibility to gastric cancer. Journal of oncology 2020; 2020: 4103264. [DOI:10.1155/2020/4103264]
8. Fesharaki SN, Naghiyan Fesharaki S, Esmaeili A, Azadeh M, Ghaedi M. SNP rs1803622 in hsa-miR-548g binding site at GAPDH alters susceptibility to breast cancer. Gene Reports 2020; 21: 100845. [DOI:10.1016/j.genrep.2020.100845]
9. Ding Gw, Gu X, Dai Z, Pan HW, Wang XY, Zhang H, Fan Y. Association of genetic polymorphisms in FOXA1 with the progression of genetic susceptibility to gastric cancer. Gastroenterology research and practice 2020; 2020: 3075837. [DOI:10.1155/2020/3075837]
10. Suhaimi SA, Chan SC, Rosli R. Matrix metallopeptidase 3 polymorphisms: Emerging genetic markers in human breast cancer metastasis. Journal of breast cancer 2020; 23(1): 1-9. [DOI:10.4048/jbc.2020.23.e17]
11. Gharazi H, Tabatabaeian H, Ghaedi K, Houshmand M, Azadeh M. Positive association of rs1049694 allele G located in NDRG1 with the incidence of gastric cancer and metastasis. Gene reports 2020; 19: 100646. [DOI:10.1016/j.genrep.2020.100646]
12. Neininger K, T Marschall, V Helms. SNP and indel frequencies at transcription start sites and at canonical and alternative translation initiation sites in the human genome. PloS one 2019; 14(4): e02148. [DOI:10.1371/journal.pone.0214816]
13. Li, C., et al., Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer. PLoS one 2014. 9(11): e112005. [DOI:10.1371/journal.pone.0112005]
14. Yamazaki T, Liu L, Lazarev D, Al-Zain A, Fomin V, Luk Yeung P, Chambers SM, Lu C-W, Studer L, Manley JL. TCF3 alternative splicing controlled by hnRNP H/F regulates E-cadherin expression and hESC pluripotency. Genes and development 2018; 32(17-18): 1161-1174. [DOI:10.1101/gad.316984.118]
15. Slyper M, Shahar A, Bar-Ziv A, Granit PZ, Hamburger T, Maly B, Peretz T, Ben-Porath I. Control of breast cancer growth and initiation by the Stem cell-associated transcription factor TCF3. Cancer research 2012; 72(21): 5613-5624. [DOI:10.1158/0008-5472.CAN-12-0119]
16. Sagara N, Katoh M. Mitomycin C resistance induced by TCF-3 overexpression in gastric cancer cell line MKN28 is associated with DT-diaphorase down-regulation. Cancer research 2000; 60(21): 5959-5962.
17. Ding HX, Lv Z, Yuan Y, Xu Q. MiRNA polymorphisms and cancer prognosis: A systematic review and meta-analysis. Frontiers in oncology 2018; 8: 596. [DOI:10.3389/fonc.2018.00596]
18. Wu M, Li X, Liu Q, Xie Y, Yuan J, Wanggou S. miR-526b-3p serves as a prognostic factor and regulates the proliferation, invasion, and migration of glioma through targeting WEE1. Cancer management and research 2019; 11: 3099. [DOI:10.2147/CMAR.S192361]
19. Han L, Liu S, Liang J, Guo Y, Shen S, Guo X, Dong Z, Guo W. A genetic polymorphism at miR‐526b binding‐site in the lincRNA‐NR_024015 exon confers risk of esophageal squamous cell carcinoma in a population of North China. Molecular carcinogenesis 2017; 56(3): 960-971. [DOI:10.1002/mc.22549]
20. Zhang ZY, Fu SL, Xu SQ, Zhou X, Liu XS, Xu SJ, Zhao IP, Wei SH. By downregulating Ku80, hsa-miR-526b suppresses non-small cell lung cancer. Oncotarget 2015; 6(3): 1462. [DOI:10.18632/oncotarget.2808]
21. Deng, N, Zhou H, Fan H, Yuan Y. Single nucleotide polymorphisms and cancer susceptibility. Oncotarget 2017; 8(66): 110635. [DOI:10.18632/oncotarget.22372]
22. Kristensen LS, Dobrovic A. Direct genotyping of single nucleotide polymorphisms in methyl metabolism genes using probe-free high-resolution melting analysis. Cancer epidemiology and prevention biomarkers 2008; 17(5): 1240-1247. [DOI:10.1158/1055-9965.EPI-07-2531]
23. Huynh LH, Bui PTK, Nguyen TT, Nguyen HT. Developing a high resolution melting method for genotyping and predicting association of SNP rs353291 with breast cancer in the Vietnamese population. Biomedical research and therapy 2017; 4(12): 1812-1831. [DOI:10.15419/bmrat.v4i12.387]
24. Ma J, Wang XB, Li R, Xuan SH, Wang F, Li XH, Zhang ZP, Tan L, Li L. RNAi-mediated TCF-3 gene silencing inhibits proliferation of Eca-109 esophageal cancer cells by inducing apoptosis. Bioscience reports 2017;37(6). [DOI:10.1042/BSR20170799]
25. Patel D, Chinaranagari S, Chaudhary J. Basic helix loop helix (bHLH) transcription factor 3 (TCF3, E2A) is regulated by androgens in prostate cancer cells. American journal of cancer research 2015; 5(11): 3407.
26. Malhotra P, Read GH, Weidhaas JB. Breast cancer and miR-SNPs: The importance of miR germ-line genetics. Non-coding RNA 2019; 5(1): 27. [DOI:10.3390/ncrna5010027]
27. Wang C, Zhao Y, Ming Y, Zhao S, Guo Z. A polymorphism at the microRNA binding site in the 3'-untranslated region of C14orf101 is associated with the risk of gastric cancer development. Experimental and therapeutic medicine 2016; 12(3): 1867-72. [DOI:10.3892/etm.2016.3521]
28. Sha Z, Lai R, Zhang X, Zhao Y, Wu J, Geng C, Guo Z. A Polymorphism at the microRNA binding Site in the 3′ Untranslated Region of KRT81 Is Associated with Breast Cancer. DNA and cell biology 2020; 39(10): 1886-94. [DOI:10.1089/dna.2019.5179]
29. Brooks AC, DeMartino AM, Brainard RE, Brittian KR, Bhatnagar A, Jones SP. Induction of activating transcription factor 3 limits survival following infarct-induced heart failure in mice. American journal of physiology, heart and circulatory physiology 2015; 309(8): 1326-35. [DOI:10.1152/ajpheart.00513.2015]
30. Kumar S, Sharma R, Garcia M, Kamel J, McCarthy C, Muth A, Phanstiel IVO. Chemoselective amide formation using O-(4-nitrophenyl) hydroxylamines and pyruvic acid derivatives. The journal of organic chemistry 2012; 77(23): 10835-10845. [DOI:10.1021/jo302175g]
31. Liu Q, Zhou JP, Li B, Huang ZC, Dong HY, Li GY, Zhou K, Nie SL. Basic transcription factor 3 is involved in gastric cancer development and progression. World journal of gastroenterology 2013; 19(28): 4495. [DOI:10.3748/wjg.v19.i28.4495]

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