Volume 25, Issue 1 (1-2021)                   ibj 2021, 25(1): 21-32 | Back to browse issues page


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Afsharnezhad M, Shahangian S S, Rasti B, Faezi Ghasemi M. Inhibitory Potential of Acroptilon repens against Key Enzymes involved in Alzheimer and Diabetes, Phytochemical Profile, Radical Scavenging, and Antibacterial Activity. ibj. 2021; 25 (1) :21-32
URL: http://ibj.pasteur.ac.ir/article-1-3231-en.html
Abstract:  
Background: This study was devoted to assessing the inhibitory potential of acetone, methanol, and ethanol extracts of Acroptilon repens against disease-associated enzymes, as well as their antioxidant/antibacterial activity and phytochemical composition. Methods: Comparative assessment using various antioxidant evaluation methods, including ferric reducing antioxidant power, scavenging ability on 2,2-diphenyl-1-picrylhydrazyl radical and hydrogen peroxide, and reducing power, indicated that the acetone extract presented the highest antioxidant activity, due to its highest total antioxidant content. Results: The total phenolic content and total flavonoids content of these extracts were 3.44 ± 0.32 mg GAE/g DW and 2.09 ± 0.2 mg QE/g DW, respectively. The hydrodistillation essential oil from A. repens was analyzed by gas chromatography-mass spectroscopy, and 17 compounds were identified. All extracts showed good inhibitory activities against disease-related enzyme acetylcholinesterase and α-amylase, with the lowest IC50 for acetonic extract. Extracts of A. repens exhibited inhibiting activities against the Gram-positive bacteria, with the most effect of acetone extract. Conclusion: Our findings suggest A. repens as a promising source of natural antioxidant, antimicrobial, anti-cholinesterase and anti-amylase agents for the management of oxidative damage, and pharmaceutical, food, and cosmeceutical purposes.
Type of Study: Full Length | Subject: Enzymology and Protein Chemistry

References
1. Sarikurkcu C, Ozer MS, Tepe B, Dilek E, Ceylan O. Phenolic composition, antioxidant and enzyme inhibitory activities of acetone, methanol and water extracts of Clinopodium vulgare L. subsp. vulgare L. Industrial crops and products 2015; 76: 961-966. [DOI:10.1016/j.indcrop.2015.08.011]
2. Shin SA, Moon SY, Kim WY, Paek SM, Park HH. Structure-based classification and anti-cancer effects of plant metabolites. International journal of molecular sciences 2018; 19(9): 2651. [DOI:10.3390/ijms19092651]
3. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World allergy organiztion journal 2012; 5(1): 9-19. [DOI:10.1097/WOX.0b013e3182439613]
4. Chalamaiah M, Dinesh Kumar B, Hemalatha R, Jyothirmayi T. Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: a review. Food chemistry 2012; 135(4): 3020-3038. [DOI:10.1016/j.foodchem.2012.06.100]
5. Girgih AT, He R, Hasan FM, Udenigwe CC, Gill TA, Aluko RE. Evaluation of the in vitro antioxidant properties of a cod (gadus morhua) protein hydrolysate and peptide fractions. Food chemistry 2015; 173: 652-659. [DOI:10.1016/j.foodchem.2014.10.079]
6. Farvin KHS, Andersen LL, Nielsen HH, Jacobsen C, Jakobsen G, Johansson I, Jessen F. Antioxidant activity of cod (gadus morhua) protein hydrolysates: in vitro assays and evaluation in 5% fish oil-in-water emulsion. Food chemistry 2014; 149: 326-334. [DOI:10.1016/j.foodchem.2013.03.075]
7. Saha S, Verma R. Inhibitory potential of traditional herbs on α-amylase activity. Pharmaceutical biology 2012; 50(3): 326-331. [DOI:10.3109/13880209.2011.608075]
8. Lee SH, Sancheti SA, Bafna MR, Sancheti SS, Seo SY. Acetylcholineterase inhibitory and antioxidant properties of rhododendron yedoense var. Poukhanense bark. Journal of medicinal plants 2011; 5(2): 248-254.
9. Pokorný J, Korczak J. Preparation of natural antioxidants in food (Pokorney J., Yanishlieva N., Gordon M eds.). London: Woodhead Publishing; 2001. [DOI:10.1201/9781439823057.ch13]
10. Hayouni EA, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of tunisian Quercus coccifera L. and Juniperus phoenicea L. Fruit extracts. Food chemistry 2007; 105(3): 1126-1134. [DOI:10.1016/j.foodchem.2007.02.010]
11. Goslee SC, Peters DPC, Beck KG. Modeling invasive weeds in grasslands:The role of allelopathy in Acroptilon repens invasion. Ecological modelling 2001; 139(1): 31-45. [DOI:10.1016/S0304-3800(01)00231-9]
12. Akihisa T, Wijeratne EMK, Tokuda H, Enjo F, Toriumi M, Kimura Y, Koike K, Nikaido T, Tezuka Y, Nishino H. Eupha-7,9(11),24-trien-3β-ol ('Antiquol C') and other triterpenes from Euphorbia antiquorum latex and their inhibitory effects on Epstein-Barr virus activation. Journal of natural products 2002; 65(2): 158-162 . [DOI:10.1021/np010377y]
13. Tunalier Z, Candan NT, Demirci B, Husnu Can Baser K. The essential oil composition of Acroptilon repens (L.) DC. of turkish origin. Flavour and fragrance journal 2006; 21: 462-464. [DOI:10.1002/ffj.1670]
14. Norouzi-Arasi H, Yavari I, Chalabian F, Kiarostami V, Ghaffarzadeh F, Nasirian A. Chemical constituents and antimicrobial activities of the essential oil of Acroptilon repens (L.) DC. Flavour and fragrance journal 2006; 21(2): 247-249. [DOI:10.1002/ffj.1568]
15. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in burkina fasan honey, as well as their radical scavenging activity. Food chemistry 2005; 91(3): 571-577. [DOI:10.1016/j.foodchem.2004.10.006]
16. Chang CC, Yang MH, Wen HM. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis 2002; 10(3): 178-182. [DOI:10.38212/2224-6614.2748]
17. Mita S, Murano N, Akaike M, Nakamura K. Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and on the accumulation of anthocyanin that are inducible by sugars. Plant journal 1997; 11(4): 841-851. [DOI:10.1046/j.1365-313X.1997.11040841.x]
18. Miliauskas G, Venskutonis PR, van beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food chemistry 2004; 85: 231-237. [DOI:10.1016/j.foodchem.2003.05.007]
19. Wijekoon MMJO, Bhat R, Karim AA. Effect of extraction solvents on the phenolic compounds and antioxidant activities of bunga kantan (Etlingera elatior Jack.) inflorescence. Journal of food composition and analysis 2011; 24(4-5): 615-619. [DOI:10.1016/j.jfca.2010.09.018]
20. Boulekbache-Makhlouf L, Medouni L, Medouni-Adrar S, Arkoub L, Madani K. Effect of solvents extraction on phenolic content and antioxidant activity of the byproduct of eggplant. Industrial cropsand products 2013; 49: 668-674 . [DOI:10.1016/j.indcrop.2013.06.009]
21. Hseu YC, Chang WH, Chen CS, Liao JW, Huang CJ, Lu FJ, Chia YC, Hsu HK, Wu JJ, Yang HL. Antioxidant activities of toona sinensis leaves extracts using different antioxidant models. Food and chemical toxicology 2008; 46(1): 105-514. [DOI:10.1016/j.fct.2007.07.003]
22. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 1976; 72: 248-254. [DOI:10.1016/0003-2697(76)90527-3]
23. Ellman GL, Courtney KD, Andres Jr. V, Feather-
24. stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology 1961; 7: 88-95. [DOI:10.1016/0006-2952(61)90145-9]
25. Mirza M, Shahmir F, Nik ZB. Chemical composition of essential oil from Acroptilon repens (L.) DC. Flavour and fragrance journal 2005; 20(6): 615-616. [DOI:10.1002/ffj.1501]
26. Razavi SM, Narouei M, Majrohi AA, Mohammaddust Chamanabad HR. Chemical constituents and phytotoxic activity of the essential oil of Acroptilon repens (L.) DC from Iran. Journal of essential oil bearing plants 2012; 15(6): 943-948. [DOI:10.1080/0972060X.2012.10662597]
27. Chavan MJ, Wakte PS, Shinde D. Analgesic and anti-inflammatory activity of Caryophyllene oxide from Annona squamosa L. bark. Phytomedicine 2010; 17(2): 149-151. [DOI:10.1016/j.phymed.2009.05.016]
28. Ghaffari T, Kafil HS, Asnaashari S, Farajnia S, Delazar A, Baek SC, Hamishehkar H, Kim KH. Chemical composition and antimicrobial activity of essential oils from the aerial parts of Pinus eldarica grown in Northwestern Iran. Molecules 2019; 24(17): 3203. [DOI:10.3390/molecules24173203]
29. Park KR, Nam D, Yun HM, Lee SG, Jang HJ, Sethi G, Cho SK, Ahn KS. β-caryophyllene oxide inhibits growth and induces apoptosis through the suppression of PI3k/AKT/mTOR/S6K1 pathways and ROS-mediated MAPKs activation. Cancer letters 2011; 312(2):178-188. [DOI:10.1016/j.canlet.2011.08.001]
30. Sitarek P, Rijo P, Garcia C, Skała E, Kalemba D, Białas AJ, Szemraj J, Pytel D, Toma M, Wysokińska
31. H, Śliwiński T . Antibacterial, anti-inflammatory, antioxidant, and antiproliferative properties of essential oils from hairy and normal roots of Leonurus sibiricus L. and their chemical composition. Oxidative medicine and cellular longevity 2017; 2017: 7384061. [DOI:10.1155/2017/7384061]
32. Dahham SS, Tabana YM, Iqbal MA, Ahamed MB, Ezzat MO, Majid ASA, Majid AMSA. The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna. Molecules 2015; 20(7):11808-11829. [DOI:10.3390/molecules200711808]
33. Rebey IB, Bourgou S, Ben Slimen Debez I, Jabri Karoui I, Hamrouni Sellami I, Msaada K, Limam F, Marzouk B. Effects of extraction solvents and provenances on phenolic contents and antioxidant activities of cumin (Cuminum cyminum L.) seeds. Food bioprocess technologyl 2012; 5: 2827-2836. [DOI:10.1007/s11947-011-0625-4]
34. Chen XM, Tait AR, Kitts DD. Flavonoid composition of orange peel and its association with antioxidant and anti-inflammatory activities. Food chemistry 2017; 218: 15-21. [DOI:10.1016/j.foodchem.2016.09.016]
35. Sulaiman SF, Sajak AAB, Ooi KL, Supriatno, Seow em. Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables. Journal of food compositionand analysisi 2011; 24(4-5): 506-515. [DOI:10.1016/j.jfca.2011.01.020]
36. Zieliński H, Kozłowska H. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. Journal of agricultural and food chemistry 2000; 48(6): 2008-2016. [DOI:10.1021/jf990619o]
37. Boeing JS, Barizão EO, Silva BCE, Montanher PF, de Cinque Almeida V, Visentainer JV. Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chemistry central journal 2014; 8(1): 48. [DOI:10.1186/s13065-014-0048-1]
38. Naczk M, Shahidi F. Phenolics in cereals, fruits and vegetables: occurrence, extraction and analysis. Journal of pharmceutical and biomedical analysis 2006; 41(5): 1523-1542. [DOI:10.1016/j.jpba.2006.04.002]
39. Noipa T, Srijaranai S, Tuntulani T, Ngeontae W. New approach for evaluation of the antioxidant capacity based on scavenging DPPH free radical in micelle systems. Food research international 2011; 44(3): 798-806. [DOI:10.1016/j.foodres.2011.01.034]
40. Zhao H, Dong J, Lu J, Chen J, Li Y, Shan L, Lin Y, Fan W, Gu G. Effects of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.) Journal of agricultural and food chemistry 2006; 54(19): 7277-7286. [DOI:10.1021/jf061087w]
41. Kchaou W, Abbès F, Blecker C, Attia H, Besbes S. Effects of extraction solvents on phenolic contents and antioxidant activities of Tunisian date varieties (Phoenix dactylifera L.). Industrial crops and products 2013; 45: 262-269. [DOI:10.1016/j.indcrop.2012.12.028]
42. Moon JK, Shibamoto T. Antioxidant assays for plant and food components antioxidant assays for plant and food components. Journal of agricultural and food chemistry 2009; 57(5): 1655-1666. [DOI:10.1021/jf803537k]
43. Alothman M, Bhat R, Karim AA. Antioxidant capacity and phenolic content of selected tropical fruits from malaysia, extracted with different solvents. Food chemistry 2009; 115: 785-788. [DOI:10.1016/j.foodchem.2008.12.005]
44. Moure A, Cruz JM, Franco D, Domõânguez JM, Sineiro J, Domõânguez H, Núñez MJ, Parajó JC. Natural antioxidants from residual sources. Food chemistry 2001; 72(2): 145-171. [DOI:10.1016/S0308-8146(00)00223-5]
45. Metrouh-Amir H, Duarte CMM, Maiza F. Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens. Industrial crops and products 2015; 67: 249-256. [DOI:10.1016/j.indcrop.2015.01.049]
46. Salehi P, Asghari B, Esmaeili MA, Dehghan H, Ghazi I. α-glucosidase and α-amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for diabetes. Journal of medicinal plants research 2013; 7: 257-266.
47. Sales PM, Souza PM, Simeoni LA, Silveira D. α-amylase inhibitors: a review of raw material and isolated compounds from plant source. Journal of pharmacy and pharmaceutical sciences 2012; 15(1): 141-183 [DOI:10.18433/J35S3K]
48. Howes MJR, Perry NSL, Houghton PJ. Plants with traditional uses and activities, relevant to the management of Alzheimer's disease and other cognitive disorders. Phytotherapy research 2003; 17(1): 1-8. [DOI:10.1002/ptr.1280]
49. Rodríguez-Vaquero MJ, Aredes-fernández PA, de Nadra MCM. Phenolic compounds from wine as natural preservatives of fish meat. Food technology and biotechnology 2013; 51(3): 376-382.
50. Ahumada-Santos YP, Montes-Avila J, de Jesús Uribe-Beltrán MDE J, PázDíaz-Camacho S, López-Angulo G, Vega-Aviña R, ÁngelLópez-Valenzuela J, Heredia JB, Delgado-Vargas F. Chemical characterization, antioxidant and antibacterial activities of six Agave species from Sinaloa, Mexico. Industrial crops and products 2013; 49: 143-149. [DOI:10.1016/j.indcrop.2013.04.050]
51. Scalbert A. Antimicrobial properties of tannins. Phytochemistry 1991; 30(12): 3875-3883. [DOI:10.1016/0031-9422(91)83426-L]
52. Humeera N, Kamili AN, Bandh SA, Amin SU, Lone BA, Gousia N. Antimicrobial and antioxidant activities of alcoholic extracts of Rumex dentatus L. Microbial pathogenesis 2013; 57:17-20. [DOI:10.1016/j.micpath.2013.02.001]
53. Cowan MM. Plant products as antimicrobial agents. Clinical microbiology reviews 1999; 12(4): 564-582. [DOI:10.1128/CMR.12.4.564]
54. Zengin G, Llorent-Martínez EJ, Córdova F, Babak MB, Mocan A, Locatelli M, Aktumsek A. Industrial crops and products. Chemical composition and biological activities of extracts from three Salvia species: S. blepharochlaena, S. euphratica var. leiocalycina, and S. verticillata subsp. amasiaca. Amasiaca. Industrial crops and products 2018; 111: 11-21. [DOI:10.1016/j.indcrop.2017.09.065]
55. Sarikurkcu C, Ozer MS, Calli N, Popović-Djordjević J. Essential oil composition and antioxidant activity of endemic Marrubium parviflorum subsp. oligodon. Industrial crops and products 2018; 119: 209-213. [DOI:10.1016/j.indcrop.2018.04.023]
56. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Journal of agricultural and food chemistry 2001; 49(11): 5165-5170. [DOI:10.1021/jf010697n]

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