Volume 25, Issue 4 (7-2021)                   IBJ 2021, 25(4): 303-307 | Back to browse issues page

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Mardi A, Biglari A, Nejatbakhsh R, Abdanipour A. Valproic Acid Ameliorates Locomotor Function in the Rat Model of Contusion via Alteration of Mst1, Bcl-2, and Nrf2 Gene Expression. IBJ 2021; 25 (4) :303-307
URL: http://ibj.pasteur.ac.ir/article-1-3161-en.html
Valproic Acid Ameliorates Locomotor Function in the Rat Model of Contusion via Alteration of Mst1, Bcl-2, and Nrf2 Gene Expression
Ali Mardi , Alireza Biglari , Reza Nejatbakhsh , Alireza Abdanipour *
Abstract:  
Background: In animal models of inflammatory diseases, Mammalian sterile 20-like kinase 1 (Mst1) facilitates the programmed cell death as a novel pro-apoptotic kinase. This research aimed to determine the expression level of Mst1 gene in a rat model of SCI treated with valproic acid (VPA). Methods: Severe rat model contusion was used for evaluation of the neuroprotective effect of valproic acid. The Basso-Beattie-Bresnahan test, was performed to determine locomotor functions. Hematoxylin/eosin staining and TUNEL assay were performed to detect cavity formation and apoptosis, respectively. The mRNA levels of the genes Mst1, nuclear factor (erythroid-derived 2)-like 2, and B-cell lymphoma 2 were evaluated, using quantitative real-time PCR acute spinal cord injury (RT-PCR). Results: The results revealed that Mst1 gene expression and TUNEL-positive cells in the VPA-treated group were significantly reduced as compared to the untreated group (p ≤ 0.05). Conclusion: Our findings indicate that VPA has therapeutic potential and can be a candidate for the treatment of neurodegenerative disorders and traumatic injury as a promising drug.
Keywords: Bcl-2, Contusion, Mst1, Nrf2, Valproic acid
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Type of Study: Short Communication | Subject: Related Fields
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