Volume 24, Issue 6 (11-2020)                   IBJ 2020, 24(6): 399-404 | Back to browse issues page

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Mousavi-Nasab S D, Sabahi F, Kaghazian H, Paryan M, Mirab Samiee S, Ghaderi M, et al . A Real-Time RT-PCR Assay for Genotyping of Rotavirus. IBJ 2020; 24 (6) :399-404
URL: http://ibj.pasteur.ac.ir/article-1-3158-en.html
Background: HRV is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on reverse transcription PCR for rapid, specific and sensitive genotyping of rotaviruses. This study describes an alternative real-time PCR assay for genotyping of rotavirus. Methods: The samples of stools studied in this research have been collected from patients referred to Children's Medical Centers, Tehran, Iran. Rotavirus detection and genotyping were performed using the RT-PCR and semi-nested RT-PCR, respectively. Samples were then genotyped with a new real-time PCR. Results: The real-time PCR was able to genotype all positive samples with a mean Ct of 28.2. Besides, a concordance rate of 100% was detected between real-time PCR and semi-nested RT-PCR. Conclusion: In this study, the genotyping of rotavirus with real-time PCR showed that this method can provide several favorable features, including high sensitivity and specificity, and a wide dynamic range for rotavirus genotyping.

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