Volume 24, Issue 5 (9-2020)                   IBJ 2020, 24(5): 333-339 | Back to browse issues page

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Mashayekhi P, Noruzinia M, Khodaverdi S. Deregulation of Stemness-Related Genes in Endometriotic Mesenchymal Stem Cells: Further Evidence for Self-Renewal/Differentiation Imbalance. IBJ 2020; 24 (5) :333-339
URL: http://ibj.pasteur.ac.ir/article-1-3098-en.html
Deregulation of Stemness-Related Genes in Endometriotic Mesenchymal Stem Cells: Further Evidence for Self-Renewal/Differentiation Imbalance
Parisa Mashayekhi , Mehrdad Noruzinia * , Sepideh Khodaverdi
Abstract:  
Background: Any irregularities in self-renewal/differentiation balance in endometriotic mesenchymal stem cells (MSCs) can change their fate and function, resulting in endometriosis development. This study aimed to evaluate the expression of OCT4 transcripts (OCT4A, OCT4B, and OCT4B1), SOX2, and NANOG in endometriotic MSCs to show their aberrant expression and to support self-renewal/differentiation imbalance in these cells. Methods: MSCs were isolated from three endometriotic and three normal endometrium samples and characterized and analyzed for the expressions of OCT4A, OCT4B, OCT4B1, SOX2, and NANOG using the quantitative real-time PCR. Results: The expressions of OCT4 transcripts and NANOG increased significantly in endometriotic MSCs, whereas SOX2 expression did not show any significant difference. Conclusion: Our findings provide further evidence for confirming the self-renewal/ differentiation imbalance in endometriotic MSCs, as the main underlying cause of endometriosis development. This study also paves the way for further research on endometriosis treatment by focusing on endometriotic stem cells.
Keywords: Endometriosis, Mesenchymal stem cells, Transcription factors
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Type of Study: Short Communication | Subject: Molecular Genetics & Genomics
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