Volume 24, Issue 4 (7-2020)                   IBJ 2020, 24(4): 236-242 | Back to browse issues page

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Shapourzadeh A, Atyabi S M, Irani S, Bakhshi H. Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber. IBJ 2020; 24 (4) :236-242
URL: http://ibj.pasteur.ac.ir/article-1-3046-en.html
Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
Atena Shapourzadeh , Seyed Mohammad Atyabi * , Shiva Irani , Hadi Bakhshi
Abstract:  
Background: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. Methods: In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. Results: The results of Fourier Transform Infrared Spectroscopy confirmed CMC (20%) graft on PCL scaffold. The Thiazolyl blue tetrazolium bromide assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of adipose-derived stem cells (ADSCs) to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. Conclusion: Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time. 
Keywords: Carboxymethyl chitosan, Mesenchymal stem cells, Tissue engineering
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Type of Study: Full Length/Original Article | Subject: Tissue Engineering and Cell Biology
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