Volume 24, Issue 5 (9-2020)                   IBJ 2020, 24(5): 281-287 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Putri Umizah L, Wasityastuti W, Indah Widasari D, Purwono S. Effects of Yacon on Colonic IFN-γ and Goblet Cells of 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model. IBJ 2020; 24 (5) :281-287
URL: http://ibj.pasteur.ac.ir/article-1-3017-en.html
Effects of Yacon on Colonic IFN-γ and Goblet Cells of 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model
Larasti Putri Umizah , Widya Wasityastuti , Dewiyani Indah Widasari , Setyo Purwono
Abstract:  
Background: Inflammatory bowel disease is a chronic inflammatory condition associated with damage to the intestinal mucosal barrier. Supplementation of yacon tubers has been known to have positive effect on intestinal health. Therefore, we conducted the present study to investigate the effect of yacon tuber powder on Th1 activation pathway by evaluating IFN-γ levels and the number of goblet cells in the colon of colitis mouse models. Methods: Thirty BALB/c mice were divided into five groups: 1, control group (G1) and 2-5, TNBS-induced colitis groups (GII-GV). Yacon powder was given to three of the TNBS-induced colitis groups with the doses of 0.165 g/30 g BW (GIII), 0.331 g/30 g body weight (BW; GIV), and 0.662 g/30 g BW (GV) for 14 days. IFN-γ levels were assessed using ELISA, while number of goblet cells was calculated based on histological observation. Results: Significantly lower IFN-γ levels was observed in GV compared to colitis group (GII) (p = 0.007). GV also showed significantly higher number of goblet cells per 100 epithelial cells in 20 crypts (p = 0.000) than GII. Conclusion: The administration of yacon powder at a dose of 0.662 g/30 g BW could decrease IFN-γ levels and improve the healing of intestinal mucosa in colitis mouse models by increasing the number of goblet cells.
Keywords: Anti-Inflammatory, Colitis, Goblet cells, IFN-γ, Supplementation
Full-Text [PDF 800 kb]      
Type of Study: Full Length/Original Article | Subject: Related Fields
References
1. Okamoto R, Watanabe M. Role of epithelial cells in the pathogenesis and treatment of inflammatory bowel disease. World journal of gastroenterology 2016; 51(1): 11-21. [DOI:10.1007/s00535-015-1098-4]
2. Kim DH, Cheon JH. Pathogenesis of inflammatory bowel disease and recent advances in biologic therapies. Immune network 2017; 12(1): 25-40. [DOI:10.4110/in.2017.17.1.25]
3. Chan JM, Bhinder G, Sham HP, Ryz N, Huang T, Bergstrom KS, Vallance BA. CD4+ T cells drive goblet cell depletion during citrobacter rodentium infection. Infection and immunity 2013; 81(12): 4649-4658. [DOI:10.1128/IAI.00655-13]
4. Steinhoff U, Visekruna A. Mucosal immunity and inflammation. Methods in microbiology 2010; 37: 353-367. [DOI:10.1016/S0580-9517(10)37015-2]
5. Neurath MF, Fuss I, Kelsall BL, Stüber E, Strober W. Antibodies to interleukin 12 abrogate established experimental colitis in mice. Journal of experimental medicine 1995; 182(5): 1281-1290. [DOI:10.1084/jem.182.5.1281]
6. Palatka K, Serfozo Z, Veréb Z, Hargitay Z, Lontay B, Erdodi F, Bánfalvi G, Nemes Z, Udvardy M, Altorjay I. Changes in the expression and distribution of the inducible and endothelial nitric oxide synthase in mucosal biopsy specimens of inflammatory bowel disease. Scandinavian journal of gastroenterology 2005; 40(6): 670-680. [DOI:10.1080/00365520510015539]
7. Bastaki SMA, Al Ahmed MM, Zaabi AA, Amir N, Adeghate, E. Effect of turmeric on large intestine histology, body weight, ulcer, IL-23, MPO and glutathione in acetic-acid-induced inflammatory bowel disease in rats. BMC complementary and alternative medicine 2016; 16: 72. [DOI:10.1186/s12906-016-1057-5]
8. Caetano BF, de Moura NA, Almeida AP, Dias MC, Sivieri K, Barbisan LF. Yacon (Smallanthus sonchifolius) as a food supplement: health-promoting benefits of fructooligosaccharides. Nutrients 2016; 8(7): pii: E436. [DOI:10.3390/nu8070436]
9. Bonet MEB, de Moreno de LeBlanc A, Dogi CA, Chaves S, Kortsarz A, Grau A, Perdigón G. Prebiotic effect of yacon (Smallanthus sonchifolius) on intestinal mucosa using a mouse model. Food and agricultural immunology 2010; 21(2): 175-189. [DOI:10.1080/09540100903563589]
10. Delgadoa GT, Thoméb R, Gabriel DL, Tamashirob WM, Pastore GM. Yacon (Smallanthus sonchifolius)-derived fructooligosaccharides improves the immune parameters in the mouse. Nutrition research 2012; 32(11): 884-892. [DOI:10.1016/j.nutres.2012.09.012]
11. Genta SB, Cabrera WM, Grau A, Sánchez SS. Subchronic 4-month oral toxicity study of dried Smallanthus sonchifolius (yacon) roots as a diet supplement in rats. Food and chemical toxicology 2005; 43(11): 1657-1665. [DOI:10.1016/j.fct.2005.05.007]
12. Lynch MJ, Raphael SS, Mellor LD, Spare PD, Inwood MJH. Medical Laboratory Technology and Clinical Pathology. Philadelphia: W.B. Saunders, 1969.
13. Bergstrom KS, Guttman JA, Rumi M, Ma C, Bouzari S, Khan MA, Gibson DL, Vogl AW, Vallance BA. Modulation of intestinal goblet cell function during infection by an attaching and effacing bacterial pathogen. Infection and immunity 2008; 76(2): 796-811. [DOI:10.1128/IAI.00093-07]
14. Lachman J, Fernández EC, Orsák M. Yacon [Smallanthus sonchifolia (Poepp. et Endl.) H. Robinson] chemical composition and use - a review. Plant soil and environment 2003; 49(6): 283-290. [DOI:10.17221/4126-PSE]
15. Lescheid DW. Probiotics as regulators of inflammation: a review. Functional foods in health and disease 2014; 4(7): 299-311. [DOI:10.31989/ffhd.v4i7.2]
16. Pedreschi R, Campos D, Noratto G, Chirinos R, Cisneros-Zevallos L. Andean Yacon root (Smallanthus sonchifolius Poepp. Endl) fructooligosaccharides as a potential novel source of prebiotics. Journal of agricultural and food chemistry 2003; 51(18): 5278-5284. [DOI:10.1021/jf0344744]
17. Rastall RA, Gibson GR, Gill HS, Guarner F, Klaenhammer TR, Pot B, Rewland IR, Sanders ME. Modulation of the microbial ecology of the human colon by probiotics, prebiotics and synbiotics to enhance human health: an overview of enabling science and potential applications. FEMS microbiology and ecology 2005; 52(2): 145-152. [DOI:10.1016/j.femsec.2005.01.003]
18. Srivastava A, Singh P, Chandra N, Tiwari D, Shukla V, Tiwari A, Kumar S. Role of probiotics in control of gastrointestinal diseases. International journal of probiotics and prebiotics 2018; 13(2/3): 55-68.
19. Galdeano CM, de Moreno Leblanc A, Vinderola G, Bonet ME, Perdigón G. Proposed model: mechanisms of immunomodulation induced by probiotic bacteria. Clinical and vaccine immunology 2007; 14(5): 485-492. [DOI:10.1128/CVI.00406-06]
20. Gourbeyre P, Denery S, Bodinier M. Probiotics, prebiotics, and synbiotics: impact on the gut immune system and allergic reactions. Journal of leukocyte biology 2011; 89(5): 685-695. [DOI:10.1189/jlb.1109753]
21. de LeBlanc ADM, del Carmen S, Zurita-Turk M, Rocha CS, van de Guchte, M, Azevedo V, Miyoshi A, LeBlanc JG. Importance of IL-10 modulation by probiotic microorganisms in gastrointestinal inflammatory diseases. ISRN gastroenterology 2011; 2011: 892971. [DOI:10.5402/2011/892971]
22. Marques C, Oliveira CS, Alves S, Chaves SR, Coutinho OP, CôRte-Real M, Preto A. Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal membrane permeabilization and cathepsin D release. Cell death and disease 2000; 4: e507. [DOI:10.1038/cddis.2013.29]
23. Segain JP, de la Blétière RD, Bourreille A, Leray V, Gervois N, Rosales C, Ferrier L, Bonnet C, Blottière H, Galmiche JP. Butyrate inhibits inflammatory responses through NFkB inhibition: implications for Crohn's disease. Gut 2000; 47(3): 397-403. [DOI:10.1136/gut.47.3.397]
24. Rafa H, Amri M, Saoula H, Belkhelfa M, Medjeber O, Boutaleb A, Aftis S, Nakmouche M, Touil-Boukoffa C. Involvement of interferon-γ in bowel disease pathogenesis by nitric oxide pathway: a study in Algerian patients. Journal of interferon and cytokine research 2010; 30(9): 691-697. [DOI:10.1089/jir.2010.0012]
25. Perner A, Nordgaard I, Matzen P, Rask-Madsen J. Colonic production of nitric oxide gas in ulcerative colitis, collagenous colitis and uninflamed bowel. Scandinavian journal of gastroenterology 2002; 37(2): 183-188. [DOI:10.1080/003655202753416858]
26. Yue G, Lai PS, Yin K, Sun FF, Nagele RG, Liu X, Linask KK, Wang C, Lin KT, Wong PY. Colon epithelial cell death in 2,4,6-trinitrobenzenesulfonic acid-induced colitis is associated with increased inducible nitric-oxide synthase expression and peroxynitrite production. Journal of pharmacology and experimental therapeutics 2001; 297(3): 915-925.
27. Antoniou E, Margonis GA, Angelou A, Pikouli A, Argiri P, Karavokyros I, Papalois A, Pikoulis E. The TNBS-induced colitis animal model: An overview. Annals of medicine and surgery (London) 2016; 11: 9-15. [DOI:10.1016/j.amsu.2016.07.019]
28. Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature 2011; 473 (7347): 337-342. [DOI:10.1038/nature10098]
29. Grant TD, Specian RD. Proliferation of goblet cells and vacuolated cells in the rabbit distal colon. Anatomical record 1998; 252(1): 41-48. https://doi.org/10.1002/(SICI)1097-0185(199809)252:1<41::AID-AR5>3.0.CO;2-H [DOI:10.1002/(SICI)1097-0185(199809)252:13.0.CO;2-H]
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Biomedical Journal

Designed & Developed by : Yektaweb