Volume 22, Issue 1 (1-2018)                   ibj 2018, 22(1): 66-69 | Back to browse issues page


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Agha Amiri S, Zarei N, Enayati S, Azizi M, Khalaj V, Shahhosseini S. Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology. ibj. 2018; 22 (1) :66-69
URL: http://ibj.pasteur.ac.ir/article-1-2114-en.html
Abstract:  

Background: Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. Methods: Four variables (cell optical density at induction, IPTG concentration, induction temperature, and induction time) were tested using experimental design. Results: Our findings demonstrated that among the examined variables, only the induction time had a significant positive effect on the protein expression yield. Conclusion: Experimental design was successfully applied in this study. The optimized condition obtained in the current study can be applied in future commercial production of this novel fusion protein.


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