:: Volume 22, Number 1 (1-2018) ::
IBJ 2018, 22(1): 33-41 Back to browse issues page
Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis
Ziba Abbasi Nejat , Shirin Farahyar, Mehraban Falahati, Mahtab Ashrafi Khozani, Aga Fateme Hosseini, Azamsadat Faiazy, Masoome Ekhtiari, Saeideh Hashemi-Hafshenjani
Abstract:  

Background: Vulvovaginal candidiasis (VVC) is an important problem due to Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (Golestan Province, Iran) from May to October 2015. Samples were analyzed using conventional mycological and molecular approaches. Clinical isolates were analyzed with specific PCR using CGL primers, and the internal transcribed spacer region and the D1-D2 domain of the large-subunit rRNA gene were amplified and sequenced. Susceptibility to amphotericin B, fluconazole, itraconazole, and clotrimazole was determined by the guidelines of the Clinical and Laboratory Standard Institute. Results: In total, 35 non-albicans Candida isolates were identified from VVC patients. The isolates included 27 strains of Candida glabrata (77.1%), 5 Candida krusei (Pichia kudriavzevii) (14.3%), 2 Candida kefyr (Kluyveromyces marxianus) (5.7%), and 1 Candida lusitaniae (Clavispora lusitaniae) (2.9%). The fungicides itraconazole and amphotericin B were effective against all species. One isolate of C. glabrata showed resistance to fluconazole and clotrimazole, and 26 isolates of C. glabrata indicated dose-dependent susceptibility to fluconazole. C. lusitaniae was susceptible in a dose-dependent manner to fluconazole and resistant to clotrimazole. Conclusions: Non-albicans Candida spp. are common agents of vulvovaginitis, and C. glabrata is the most common species in the tested patients.

Keywords: Candida glabrata, Vulvovaginal candidiasis, Candida krusei
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Type of Study: Full Length | Subject: Molecular Microbiology
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DOI: 10.22034/ibj.22.1.33
PMID: 28688376


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Abbasi Nejat Z, Farahyar S, Falahati M, Ashrafi Khozani M, Hosseini A F, Faiazy A, et al . Molecular Identification and Antifungal Susceptibility Pattern of Non-albicans Candida Species Isolated from Vulvovaginal Candidiasis . IBJ. 2018; 22 (1) :33-41
URL: http://ibj.pasteur.ac.ir/article-1-2112-en.html
Volume 22, Number 1 (1-2018) Back to browse issues page
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