:: Volume 22, Issue 1 (1-2018) ::
IBJ 2018, 22(1): 22-32 Back to browse issues page
Immunization of C57BL/6 Mice with GRA2 Combined with MPL Conferred Partial Immune Protection against Toxoplasma gondii
Jalal Babaie , Samira Amiri , Robab Homayoun , Ebrahim Azimi , Reyhaneh Mohabati , Mahboobe Berizi , M. Reza Sadaie , Majid Golkar
Abstract:  

BBackground: We have previously reported that immunization with GRA 2 antigen of Toxoplasma gondii induces protective immunity in CBA /J (H2k) and BALB/c mice (H2d). We aimed to examine whether immunization of a distinct strain of rodent with recombinant dense granule antigens (GRA2) combined with monophosphorryl lipid A (MPL) adjuvant elicits protective immune response against T. gondii. Methods: C57BL/6 (H2b haplotype) mice were immunized with GRA 2, formulated in MPL adjuvant. Results: Strong humoral response, predominantly of IgG1 subclass and cellular response, IFN-γ, was detected at three weeks post immunization. Mice immunized with GRA 2 had significantly (p < 0.01) fewer brain cysts than those in the adjuvant group, upon challenge infection. Despite the production of a strong antibody response, IFN-γ production and brain cyst reduction were not significant when the immunized mice were infected four months after the immunization. Conclusions: We can conclude that GRA2 immunization partially protects against T. gondii infection in C57BL/6 mice, though the potency and longevity of this antigen as a standalone vaccine may vary in distinct genetic backgrounds. This observation further emphasizes the utility of GRA 2 for incorporation into a multi-antigenic vaccine against T. gondii.

Keywords: Immunization, GRA2, Toxoplasma, MPL
Full-Text [PDF 869 kb]      
Type of Study: Full Length | Subject: Molecular Immunology & Vaccines
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Babaie J, Amiri S, Homayoun R, Azimi E, Mohabati R, Berizi M, et al . Immunization of C57BL/6 Mice with GRA2 Combined with MPL Conferred Partial Immune Protection against Toxoplasma gondii. IBJ. 2018; 22 (1) :22-32
URL: http://ibj.pasteur.ac.ir/article-1-2095-en.html


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