Volume 20, Issue 3 (7-2016)                   ibj 2016, 20(3): 182-186 | Back to browse issues page


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Shokrollahi N, Shahbazzadeh D, Pooshang-Bagheri K, Habibi-Anbouhi M, Jahanian-Najafabadi A, Behdani M. A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System. ibj. 2016; 20 (3) :182-186
URL: http://ibj.pasteur.ac.ir/article-1-1554-en.html
Abstract:  

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. Results: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. Conclusion: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.


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