:: Volume 19, Issue 3 (7-2015) ::
IBJ 2015, 19(3): 143-148 Back to browse issues page
Association of tcdA+/tcdB+ Clostridium difficile Genotype with Emergence of Multidrug-Resistant Strains Conferring Metronidazole Resistant Phenotype
Farahnaz-Sadat Shayganmehr , Masoud Alebouyeh , Masoumeh Azimirad , Mohammad Mehdi Aslani , Mohammad Reza Zali
Abstract:  

Background: Reduced susceptibility of Clostridium difficile to antibiotics is problematic in clinical settings. There is new evidence indicating the cotransfer of toxin-encoding genes and conjugative transposons encoding resistance to antibiotics among different C. difficile strains. To analyze this association, in the current study, we evaluated the frequency of toxigenic C. difficile among the strains with different multidrug-resistant (MDR) profiles in Iran. Methods: Antimicrobial susceptibility patterns and minimal inhibitory concentrations (MIC) of the isolates were determined against metronidazole, imipenem, ceftazidime, amikacin, and ciprofloxacin by agar dilution method. The association of the resistance profiles and toxigenicity of the strains were studied by PCR targeting tcdA and tcdB genes. Results: Among 86 characterized strains, the highest and lowest resistance rates were related to ciprofloxacin (97%) and metronidazole (5%), respectively. The frequency of resistance to other antibiotics was as follow: imipenem (48%), ceftazidime (76%), and amikacin (76.5%). Among the resistant strains, double drug resistance and MDR phenotypes were detected in the frequencies of 10.4% and 66.2%, respectively. All of the metronidazole-resistant strains belonged to tcdA +/tcdB + genotype with triple or quintuple drug resistance phenotypes. MIC50 and MIC90 for this antibiotic was equally &le 8 &mug/ml. Conclusion: These results proposed the association of tcdA +/tcdB + genotype of C. difficile and the emergence of resistance strains to broad-spectrum antibiotics and metronidazole.

Keywords: Multidrug resistance, Clostridium difficile, Metronidazole
Full-Text [PDF 166 kb]      
Type of Study: Full Length | Subject: Related Fields
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shayganmehr F, Alebouyeh M, Azimirad M, Aslani M M, Zali M R. Association of tcdA+/tcdB+ Clostridium difficile Genotype with Emergence of Multidrug-Resistant Strains Conferring Metronidazole Resistant Phenotype. IBJ. 2015; 19 (3) :143-148
URL: http://ibj.pasteur.ac.ir/article-1-1413-en.html


Volume 19, Issue 3 (7-2015) Back to browse issues page
Persian site map - English site map - Created in 0.17 seconds with 37 queries by YEKTAWEB 3767